针灸推拿医学（英文版）2025，Vol.23Issue(2)：107-116,10.DOI:10.1007/s11726-025-1487-z
基于TLR4/Myd88通路探讨电针对功能性消化不良大鼠肠黏膜屏障功能的影响
Effect of electroacupuncture on intestinal mucosal barrier function in functional dyspepsia rats based on TLR4/Myd88 pathway
摘要
Abstract
Objective:To investigate the effects of electroacupuncture on duodenal inflammation and intestinal mucosal barrier function in functional dyspepsia(FD)rats based on the Toll-like receptors 4(TLR4)/myeloid differentiation factor 88(Myd88)pathway. Methods:Sixty male Sprague-Dawley rats were randomly divided into a blank group(n=10)and a modeling group(n=50).The FD model was induced in the modeling group using a multi-factor comprehensive intervention.Forty successful model rats were randomly allocated into a model(FD)group,an electroacupuncture(EA)group,a TLR4 inhibitor(TAK242)group,and an EA+TAK242 group,with 10 rats in each group.In the EA group,following acupuncture at Zusanli(ST36)and Taichong(LR3),a filiform needle was perpendicularly inserted 3 mm into the lateral side of Zusanli(ST36)bilaterally,positioned between the Stomach Meridian and the Gallbladder Meridian at the lateral edge of the tibia,serving as an auxiliary electrode.An EA instrument was then connected to Zusanli(ST36)and the auxiliary electrode on the same side for intervention.The TAK242 group received tail vein injections of TLR4 inhibitor TAK242[0.5 mg/(kg·bw)],while the EA+TAK242 group received both TAK242 injections and EA intervention.Intragastric residual rate and small intestinal propulsion rate were observed in each group.Immunohistochemistry was used to detect tryptase expression in the duodenum;enzyme-linked immunosorbent assay was used to measure serum concentrations of 5-hydroxytryptamine(5-HT)and 5-hydroxyindole acetic acid(5-HIAA);Western blotting was used to analyze TLR4,MyD88,zonula occludens-1(ZO-1),and junctional adhesion molecule A(JAM-A)protein expression in the duodenal tissue. Results:In the FD group,the intragastric residual rate increased,small intestinal propulsion rate decreased,ZO-1 and JAM-A protein expression decreased,duodenal tryptase mean optical density increased,serum 5-HT and 5-HIAA levels increased significantly,and TLR4 and MyD88 protein expression increased significantly compared to the blank group(P<0.01).Compared to the FD group,the EA,TAK242,and EA+TAK242 groups showed a decreased gastric residual rate,increased small intestinal propulsion rate,increased ZO-1 and JAM-A protein expression,decreased optical density of duodenal tryptase,and significantly reduced serum 5-HT and 5-HIAA,as well as decreased TLR4 and MyD88 protein levels(P<0.01).Compared to the EA group,the TAK242 group had a higher intragastric residual rate and lower small intestinal propulsion rate(P<0.05),decreased TLR4 protein expression(P<0.05),no significant change in MyD88 protein expression(P>0.05),increased optical density of duodenal tryptase(P<0.05),increased serum 5-HT and 5-HIAA levels(P<0.05),and decreased ZO-1 and JAM-A protein expression(P<0.05).In the EA+TAK242 group,the intragastric residual rate was significantly lower,small intestinal propulsion rate was significantly higher,TLR4 and MyD88 protein expression was significantly lower,optical density of duodenal tryptase was significantly lower,and ZO-1 and JAM-A protein expression levels were significantly higher compared to the EA and TAK242 groups(P<0.01). Conclusion:EA promotes gastrointestinal motility,restores intestinal mucosal barrier function,and reduces inflammatory responses in FD rats,potentially through the down-regulation of the TLR4/Myd88 pathway.关键词
针刺疗法/电针/功能性消化不良/肠黏膜屏障功能/Toll样受体4/大鼠Key words
Acupuncture Therapy/Electroacupuncture/Functional Dyspepsia/Intestinal Mucosal Barrier Function/Toll-like Receptor 4/Rats分类
医药卫生引用本文复制引用
康朝霞,崔冬梅,杨燕萍..基于TLR4/Myd88通路探讨电针对功能性消化不良大鼠肠黏膜屏障功能的影响[J].针灸推拿医学（英文版）,2025,23(2):107-116,10.基金项目
This work was supported by the Project of National Natural Science Foundation of China(国家自然科学基金项目,No.82060897) （国家自然科学基金项目,No.82060897）
Basic Research(Natural Science)Project of Guizhou Science and Technology Department[贵州省科技厅基础研究(自然学科)项目,No.黔科合基础-ZK(2022)一般466] （Natural Science）
Science and Technology Research Project of Traditional Chinese Medicine and Ethnic Medicine of Guizhou Provincial Administration of Traditional Chinese Medicine(贵州省中医药管理局中医药、民族医药科学技术研究项目,No.QZYY-2022-007) （贵州省中医药管理局中医药、民族医药科学技术研究项目,No.QZYY-2022-007）
Youth Project of Scientific Research Project of Higher Education Institutions of Guizhou Provincial Department of Education[贵州省教育厅高等学校科学研究项目青年项目,No.黔科技(2022)211] （2022）
Guizhou Provincial Health Commission Science and Technology Fund Project(贵州省卫生健康委科学技术基金项目,No.gzwkj2024-350) （贵州省卫生健康委科学技术基金项目,No.gzwkj2024-350）
PhD Start-up Fund of Guizhou University of Traditional Chinese Medicine[贵州中医药大学博士启动基金,No.贵中医博士启动(2020)27号]. （2020）
