中药材2024，Vol.47Issue(8)：2052-2057,6.DOI:10.13863/j.issn1001-4454.2024.08.030
黄芪甲苷通过上调miR-1226缓解ox-LDL诱导人脐静脉血管内皮细胞损伤
Effects of Astragaloside Ⅳ on ox-LDL-Induced Endothelial Cell Injury in Human Umbilical Vein by Upregulating miR-1226
摘要
Abstract
Objective:To discuss the effect of astragaloside Ⅳ on injury of human umbilical vein endothelial cells(HUVECs)and its mechanism.Methods:HUVECs were cultured in vitro and treated with 100 mg/L ox-LDL to establish atherosclerosis(As)cell mod-el.HUVECs were divided into blank control group,model group,low,medium and high doses astragaloside Ⅳ groups,miR-NC group,miR-1226 group,high dose astragaloside Ⅳ with anti-miR-NC group,high dose astragaloside Ⅳ with anti-miR-1226 group.Flow cytom-etry was used to detect apoptosis.ELISA method was used to detected IL-6,IL-1β,TNF-α levels;The protein expression was detected by Western Blot.The expression of miR-1226 was detected by RT-qPCR.Results:Compared with blank control group,apoptosis rate,Bax protein expression and the secretion of IL-6,IL-1β and TNF-α were significantly increased in model group,while expressions of Bcl-2 protein and miR-1226 were significantly decreased(P<0.05).Compared with the model group,the apoptosis rate,Bax protein expres-sion and the secretion of IL-6,IL-1β and TNF-α in each astragaloside Ⅳ concentration group and miR-1226 group were significantly in-creased,the expression of Bcl-2 protein was significantly decreased,and the expressions of Bcl-2 protein and miR-1226 were significant-ly increased(P<0.05).The effect of astragaloside Ⅳ was concentration-dependent.Down-regulation of miR-1226 reversed the effect of astragaloside Ⅳ.Conclusion:Astragaloside Ⅳ may alleviate ox-LOL-induced HUVECs injury by up-regulating the expression of miR-1226.关键词
黄芪甲苷/miR-1226/凋亡/炎性细胞因子Key words
Astragaloside Ⅳ/miR-1226/Apoptosis/Inflammatory cytokines分类
中医学引用本文复制引用
刘文频,熊向晖,宾晓芳,伍瑶,高海波,陈聪伶..黄芪甲苷通过上调miR-1226缓解ox-LDL诱导人脐静脉血管内皮细胞损伤[J].中药材,2024,47(8):2052-2057,6.基金项目
国家自然科学基金项目(81673955) （81673955）
2019年度湖南省大学生创新创业训练计划项目(S201910541011) （S201910541011）
