中药材2024，Vol.47Issue(7)：1655-1660,6.DOI:10.13863/j.issn1001-4454.2024.07.010
栝楼黄酮类生物合成关键酶基因的挖掘
Mining of Key Enzyme Genes for Flavonoid Biosynthesis of Trichosanthes kirilowii
摘要
Abstract
Objective:To explore the enzyme genes associated with the biosynthesis pathway of secondary metabolites of flavonoids from Trichosanthes kirilowii,so as to understand the regulatory mechanism of the biosynthesis of flavonoids of Trichosanthes kirilowii.Methods:The flower buds of female and male Trichosanthes kirilowii were used as materials,the differentially expressed genes(DEGs)by transcriptome sequencing,bioinformatics and gene function annotation were screened,qRT-PCR assay was used to verify the key en-zyme genes.The contents of total flavonoids in leaves,stems and flowers of female and male Trichosanthes kirilowii were determined by HPLC.The correlations between the expressions of genes and the contents of total flavonoids in different parts of Trichosanthes kirilowii were analyzed by Pearson method.Results:17 619 567 and 16 699 544 high quality reads were obtained from the female and male flow-er bud libraries,respectively.156 032 Unigenes were obtained after assembly and redundancy removal,and 25 435 DEGs were ob-tained.The KEGG metabolic pathway showed that 80 DEGs were involved in the flavonoid biosynthesis of Trichosanthes kirilowii,inclu-ding key enzyme genes such as ANR,CHS and HCT.The total flavonoid content of male Trichosanthes kirilowii was higher than that of female Trichosanthes kirilowii(P≤0.001),and the expressions of the top three key genes of flavonoid biosynthesis pathway in male Tri-chosanthes kirilowii were significantly higher than in female Trichosanthes kirilowii(P≤0.001).ANR,CHS and HCT genes showed dif-ferent correlations with total flavonoid content in male and female Trichosanthes kirilowii plants.Conclusion:The results lay a foundation for further study the regulatory role of functional genes in the metabolic pathway of Trichosanthes kirilowii and the genetic mechanism of the difference of flavonoids between male and female strains.关键词
栝楼/次生代谢/生物合成/关键酶基因/黄酮类成分Key words
Trichosanthes kirilowii Maxim./Secondary metabolism/Biosynthesis/key enzyme gene/Flavonoids分类
中医学引用本文复制引用
胡秀芹,梁秀坤,张文玉,刘言娟,辛杰,张永清..栝楼黄酮类生物合成关键酶基因的挖掘[J].中药材,2024,47(7):1655-1660,6.基金项目
国家自然科学基金项目(81803651) （81803651）
