中药材2024，Vol.47Issue(9)：2192-2197,6.DOI:10.13863/j.issn1001-4454.2024.09.009
基于叶绿体基因组SNP鉴定不同化学型的广藿香
Identification of the Different Chemotypes of Pogostemon cablin Based on Chloroplast Genome SNP
摘要
Abstract
Objective:To establish a molecular identification method for different chemotypes of Pogostemon cablin based on the variation of single nucleotide polymorphism(SNP)in chloroplast genome.Methods:According to the difference of one base insertion/de-letion in ycf3-trnS_GGA gene of chloroplast genome of different chemotypes of Pogostemon cablin,primers were designed to amplify this sequence,and different samples of Pogostemon cablin were amplified by PCR and confirmed by dideoxy sequencing.The contents of pat-choulenone and patchouli alcohol in Pogostemon cablin were determined by gas chromatography and the ratio of them was calculated.Results:A band of 196 bp could be amplified from fresh,medicinal and proprietary Chinese medicines containing Pogostemon cablin.Alcohol type Pogostemon cablin was A 130 bp of the sequence,the alcohol type Pogostemon cablin was A base,while the ketone type lacked an A base at this site,and ketone type Pogostemon cablin was missing A base at this site.The molecular identification results of sample chemotypes was consistent with the results of gas chromatography.Conclusion:The established molecular identification method based on the SNP site of chloroplast genome of Pogostemon cablin can effectively distinguish different chemotypes of Pogostemon cablin,and this method is accurate,reliable,and has the potential to be widely applied.关键词
广藿香/化学型/叶绿体基因组/单核苷酸多态性/气相色谱Key words
Pogostemon cablin(Blanco)Benth./Chemotype/Chloroplast genome/Single Necleotide Polymorphism(SNP)/Gas chromatography分类
中医学引用本文复制引用
陆亚茹,彭晓祺,吴文如,行冰楠,邹何元..基于叶绿体基因组SNP鉴定不同化学型的广藿香[J].中药材,2024,47(9):2192-2197,6.基金项目
中央本级重大增减支项目"名贵中药资源可持续利用能力建设项目"(2060302) （2060302）
广州中医药大学大学生创新创业训练计划项目(202210572232) （202210572232）
广东省科学技术厅2020年度广东省科技特派员项目(KTP20200137) （KTP20200137）
