中药材2024，Vol.47Issue(9)：2264-2271,8.DOI:10.13863/j.issn1001-4454.2024.09.021
桑白皮不去粗皮与去粗皮指纹图谱及色度值的比较研究
Comparative Study on Fingerprint and Chroma Value of Mori Cortex Containing Raw Bark and Peeling Raw Bark
摘要
Abstract
Objective:To compare the difference of Mori Cortex containing raw bark and peeling raw bark.Methods:A total of 12 batches of Mori Cortex from different habitats were collected,the fingerprints of Mori Cortex containing raw bark and peeling raw bark were established by UPLC,the similarity evaluation was carried out.The chroma values(L*,a*,b*)of each batch of Mori Cortex pow-der were determined,and the average total chroma values E* was calculated.The one-way ANOVA,principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to perform multivariate statistical analysis.Re-sults:The established fingerprints of Mori Cortex containing raw bark and peeling raw bark all had 23 common peaks,and chromato-graphic peaks 6,13,18 were identified as mulberroside A,oxyresveratrol,kuwanon G,respectively.The similarity between the finger-prints of 12 batches of Mori Cortex containing raw bark and peeling raw bark was all greater than 0.900.The results of one-way ANOVA showed that the chromatographic peaks 1,3,15 to 23 and the colorimetric values L*,a*,E* were significantly different between the fingerprints of Mori Cortex containing raw bark and peeling raw bark(P<0.05).The chromatographic peaks 2,4,7,11,14 were posi-tively correlated with colorimetric values a*,b*,while the chromatographic peak 7 was negatively correlated with L*.PCA could be di-vided into two kinds of most Mori Cortex containing raw bark and peeling raw bark.The comprehensive score of principal components of most Mori Cortex containing raw bark was higher than that of Mori Cortex peeling raw bark.According to the VIP>1,the peaks 1,3,15 to 23 were the main difference components between Mori Cortex containing raw bark and peeling raw bark.Conclusion:The established UPLC fingerprint method of Mori Cortex is stable and feasible,and there are differences in the fingerprints and chroma values of Mori Cortex containing raw bark and peeling raw bark.关键词
桑白皮/不去粗皮与去粗皮/指纹图谱/色度值/多元统计分析Key words
Mori Cortex/Containing raw bark and peeling raw bark/Fingerprint/Chroma value/Multivariate statistical analysis分类
医药卫生引用本文复制引用
刘佩仪,邓李红,王文丽,彭帮贵,刘远俊,徐杰,张正..桑白皮不去粗皮与去粗皮指纹图谱及色度值的比较研究[J].中药材,2024,47(9):2264-2271,8.基金项目
工信部2022年产业技术基础公共服务平台项目(2022-230-221) （2022-230-221）
