中药材2024，Vol.47Issue(7)：1694-1699,6.DOI:10.13863/j.issn1001-4454.2024.07.017
云南蓍乙酸乙酯部位化学成分及抗氧化活性研究
Chemical Constituents and Antioxidant Activity of Ethyl Acetate Part from Achillea wilsoniana
摘要
Abstract
Objective:To study the chemical constituents and antioxidant activity of Achillea wilsoniana.Methods:The ethyl acetate extract of 90%ethanol extract from Achillea wilsoniana was isolated and purified by silica gel,Sephadex LH-20,MCI and semi-prepara-tive high performance liquid chromatography,and the antioxidant activity of the monomer compound was tested by 96-well plate.Re-sults:Fourteen compounds were isolated from ethyl acetate extract of Achillea wilsoniana and identified as(-)-aptosimon(1),(+)-episesaminone(2),(+)-pinoresinol(3),linderin A(4),(-)-loliolide(5),madhusic acid(6),1β-hydroxy-α-cyperone(7),pubiner-noid A(8),ficusic acid(9),erythro-2,3-bis(4-hydroxy-3-methoxyphenyl)-3-ethoxypropan-1-ol(10),artemetin(11),isofraxidin(12),pellitorine(13),ethyl succinate(14),respectively.Among them,compounds 2,3,10,12 showed potent scavenging effect on DPPH free radicals with IC50 values ranging of 0.755 to 2.494 μg/mL,while compounds 1,3,6,9,11,12,14 had a pronounced inhibition rates on tyrosinase with IC50 values ranging of 0.157 to 11.803 μg/mL.Conclusion:Among them,compounds 1 to 6,8 to 10 and 14 are isolated from Achillea genus for the first time,compound 12 is isolated from Achillea wilsoniana for the first time.关键词
云南蓍/化学成分/分离鉴定/抗氧化活性Key words
Achillea wilsoniana Heimerl ex Hand.-Mazz./Chemical constituents/Isolation and identification/Antioxidant activity分类
医药卫生引用本文复制引用
王真真,尚莹,邓超凡,张慧,马晓莉,江志波,南泽东..云南蓍乙酸乙酯部位化学成分及抗氧化活性研究[J].中药材,2024,47(7):1694-1699,6.基金项目
北方民族大学科研启动基金(2021KYQD35) （2021KYQD35）
北方民族大学研究生创新项目(YCX23174,YCX23181) （YCX23174,YCX23181）
国家自然科学基金地区项目(82160672) （82160672）
宁夏自然科学基金优秀青年项目(2022AAC05041) （2022AAC05041）
