中药材2024，Vol.47Issue(7)：1706-1711,6.DOI:10.13863/j.issn1001-4454.2024.07.019
毛鸡骨草化学成分及抗炎活性研究
Chemical Constituents of Abrus mollis and Anti-inflammatory Activity
摘要
Abstract
Objective:To study the chemical constituents of Abrus mollis and anti-inflammatory activity.Methods:The methanol ex-tract of Abrus mollis was isolated and purified by column chromatography on silica gel,ODS and semi preparative-HPLC,The structures were identified by HR-ESI-MS and NMR spectroscopic data analyses and comparison with reported spectroscopic data.The inhibitory activity of the compounds on the production of nitric oxide(NO)in RAW264.7 macrophages of mice induced by lipopolysaccharide(LPS)was measured by Griess method.Results:Seventeen compounds were isolated from the ethyl acetate extract of the methanol ex-tract of Abrus mollis and identified as formononetin(1),afromosin(2),8-hydroxy-4',7-dimethoxyisoflavone(3),8-methylretusin(4),biochanin A(5),calycosin(6),7-hydroxy-3',4'-dimethoxyisoflavone(7),geraldone(8),5,7,4'-trihydroxy-3'-methoxyflavone(9),3-formylindole(10),methyl hematinate(11),methyl ferulate(12),dimethyl phthalate(13),dibutylphthalate(14),p-hydroxybenzalde-hyde(15),salicylic acid(16),methyl 4-hydroxybenzoate(17),respectively.At 20 μmol/L,compounds 5,8,9 and 12 inhibited NO pro-duction in RAW 264.7 cells induced by LPS from 79.15%to 88.93%.Conclusion:Among them,compounds 6 to 9,11 to 13 and 15 are isolated from Abrus genus for the first time,compounds 5,8,9 and 12 have potential anti-inflammatory activities.关键词
毛鸡骨草/黄酮/异黄酮/抗炎Key words
Abrus mollis Hance/Flavones/Isoflavones/Anti-inflammatory分类
医药卫生引用本文复制引用
黄志琼,陈道峰,杨世林,苑仁祎坤,黄莉婷,潘其明..毛鸡骨草化学成分及抗炎活性研究[J].中药材,2024,47(7):1706-1711,6.基金项目
"带土移植"人才引育计划(桂科AA23026010) （桂科AA23026010）
广西中医药大学-柳药集团青年科技创新能力提升计划(YXY0000305) （YXY0000305）
