中药材2024，Vol.47Issue(8)：1893-1898,6.DOI:10.13863/j.issn1001-4454.2024.08.004
化橘红枝枯病病原菌鉴定及室内杀菌剂筛选
Identification and Fungicide Screening of the Pathogen Causing Branch Rot of Citrus grandis
摘要
Abstract
Objective:To identify the pathogen causing Citrus grandis branch rot and screen for suitable fungicides.Methods:The pathogenic bacteria were identified by tissue isolation and purification,tieback pathogenicity determination,morphology and molecular biology methods,and suitable fungicides were screened by plate bacteriostatic assay.Results:A pure strain was isolated from the affected branches of Citrus grandi branch rot disease,and the pathogenicity test results showed that this strain was the pathogen of branch rot.According to morphological and molecular biology identification,the pathogen causing branch rot was Lasiodiplodia theobromae.Labora-tory drug screening revealed that the suspension of iprodione had the strongest inhibitory effect,with inhibition rates reaching 100%at different concentrations.Prochloraz,hymexxazol aqueous,dioctyldiethy lenetriamine acetate aqueous had significant inhibitory effects with EC50 values of 88.42,109.61,110.58 mg/L,respectively.The suspension of pyraclostrobin had a poor inhibitory effect on Lasio-diplodia theobromae,while the suspension of thiodiazole copper had no inhibitory effect on Lasiodiplodia theobromae.Conclusion:Lasio-diplodia theobromae is a pathogen that causes Citrus grandi branch rot.The laboratory bacteriostatic test shows that the suspension agent of iprodione and prochloraz has obvious bacteriostatic effect,which can provide reference for the field control of Citrus grandis branch rot.关键词
化橘红/枝枯病/病原菌鉴定/杀菌剂Key words
Citrus grandis'Tomentosa'/Branch rot/Pathogen identification/Fungicides分类
农业科技引用本文复制引用
何勤勤,苏雪燕,刘嘉怡,何黎明,谢春生,韩寒冰,黄新敏..化橘红枝枯病病原菌鉴定及室内杀菌剂筛选[J].中药材,2024,47(8):1893-1898,6.基金项目
2018年度广东高校科研平台和项目青年创新人才项目(2018KQNCX1632019) （2018KQNCX1632019）
2022年广东省省级乡村振兴战略专项资金种业振兴项目(2022-NPY-00-046) （2022-NPY-00-046）
2019年度广东省普通高校"服务乡村振兴计划"重点领域专项(2019KZDZX2005) （2019KZDZX2005）
2022年广东石油化工学院大学生创新训练项目(73322212) （73322212）
