中药材2024，Vol.47Issue(8)：1995-2000,6.DOI:10.13863/j.issn1001-4454.2024.08.020
代代花黄酮类成分指纹图谱研究及8个成分含量测定
Study on Fingerprint of Flavonoids in the Flower of Citrus aurantium var.amara and Determination of the Contents of 8 Components
摘要
Abstract
Objective:To establish the HPLC fingerprint of the flower of Citrus aurantium var.amara and to determine the contents of 8 flavonoids narirutin,naringin,hesperidin,neohesperidin,naringenin,hesperidin,nobiletin and tangeretin.Methods:Using Diamonsil(2)C18(250 mm×4.6 mm,5 μm)chromatographic column,the mobile phase was acetonitrile-0.1%phosphoric acid solution with gra-dient elution.The flow rate was 1.0 mL/min,detection wavelength was 320 nm,column temperature was 30℃,sample size was 10 μL.The similarity evaluation,cluster analysis(CA),principal component analysis(PCA)and orthogonal partial least square discriminant a-nalysis(OPLS-DA)of 21 batches of samples from different origin were carried out to select the quality difference components.Results:The similarity of fingerprints of 21 batches of flower of Citrus aurantium var.amara was 0.983～0.999,19 common peaks were identi-fied,8 components were identified by reference products,21 batches of flower of Citrus aurantium var.amara were divided into 2 catego-ries by CA and PCA,OPLS-DA screened out 7 components that caused the difference in the classification of Citrus aurantium var.amara flower from different places.The contents of above eight components were 2.30～10.00 mg/g,27.25～60.87 mg/g,5.61～16.31 mg/g,44.07～84.17 mg/g,0.03～0.78 mg/g,0.02～0.88 mg/g,0.05～0.14 mg/g,0.11～0.24 mg/g,respectively.Conclu-sion:The fingerprint and multi-index content determination method established in this study is accurate and reliable,and can provide a basis for the quality evaluation of Citrus aurantium var.amara flower.关键词
代代花/HPLC指纹图谱/含量测定/黄酮Key words
Flower of Citrus aurantium L.var.amara Engl./HPLC Fingerprint/Content determination/Flavonoids分类
医药卫生引用本文复制引用
左月明,曾晓梅,张忠立,高凌云..代代花黄酮类成分指纹图谱研究及8个成分含量测定[J].中药材,2024,47(8):1995-2000,6.基金项目
国家自然科学基金项目(82160928) （82160928）
