中山大学学报（医学科学版）2025，Vol.46Issue(2)：179-185,7.
支架蛋白PDLIM5增强小胶质细胞吞噬及其在多发性硬化中作用的研究
Enhancement of Microglial Phagocytosis by Scaffold Protein PDLIM5 and Its Role in Multiple Sclerosis
摘要
Abstract
[Objective]To investigate the expression of scaffold protein PDLIM5 in multiple sclerosis(MS)patients and the mouse microglial cell line BV2,and to explore its effects on the phagocytosis of microglial cells.[Methods]Peripheral blood samples were collected from 24 MS patients and 6 healthy volunteers as controls.The expression levels of PDLIM5 were detected by real-time quantitative PCR.A neuroinflammation cell model was established by treating the mouse microglial cell line BV2 with lipopolysaccharide(LPS,1 μg/mL).The expression levels of PDLIM5 were measured by Western Blot.The effect of PDLIM5 expression on phagocytosis was analyzed by transfecting BV2 cells with PDLIM5 shRNA plasmids or PDLIM5 overexpression plasmids.[Results]Real-time quantitative PCR results showed that compared with the healthy control group,the expression level of PDLIM5 from the MS patients was significantly increased in monocytes[2.78(0.70-6.86)vs.0.54(0.39-1.51),P=0.036]and lymphocytes[1.62(0.90-2.26)vs.0.11(0.05-0.21),P<0.001].Western Blot results indicated that PDLIM5 expression was significantly upregulated in BV2 cells following LPS stimulation(P<0.05).Plasmid transfection experiments demonstrated that knockdown of PDLIM5 inhibited the phagocytic capacity of BV2 cells as measured by trypan blue uptake(P<0.05),while overexpression of PDLIM5 enhanced the phagocytic ability of BV2 cells(P<0.001).[Conclusion]Under neuroinflammatory conditions,PDLIM5 expression is elevated,and this upregulation promotes the phagocytosis of microglial cell.关键词
PDLIM5/多发性硬化/小胶质细胞/细胞吞噬/神经炎症Key words
PDLIM5/multiple sclerosis/microglia/phagocytosis/neuroinflammation分类
基础医学引用本文复制引用
陈海莲,王玉鸽,崔宇,平苏宁,陈元..支架蛋白PDLIM5增强小胶质细胞吞噬及其在多发性硬化中作用的研究[J].中山大学学报（医学科学版）,2025,46(2):179-185,7.基金项目
国家自然科学基金(8230062128) （8230062128）
广东省自然科学基金(2022A1515012314,2023A1515010374) （2022A1515012314,2023A1515010374）
深圳市基础研究(JCYJ20220530145615034) （JCYJ20220530145615034）
