中山大学学报（医学科学版）2025，Vol.46Issue(2)：345-353,9.
食物过敏伴便秘儿童肠道菌群变化与功能分析
Changes and Functions Analysis of Intestinal Flora in Children with Food Allergy and Constipation
摘要
Abstract
[Objective]To investigate the changes of intestinal flora in children with food allergy and constipation by high-throughput sequencing,and to analyze the function of intestinal flora,to provide a basis for the prevention and treatment of food allergy and constipation in children.[Methods]Totally 33 children with food allergy and constipation in Longgang District of Shenzhen were selected as the disease group(CPFA group),another 59 healthy children of the same age were recruited as the HC group.Stool samples were collected and subjected to high-throughput sequencing of 16SrRNA genes,followed by bioinformatics analysis.[Results]① The species abundance of HC group was higher than that of CPFA group,and the diversity of intestinal microbial community was different between the two groups;② At the phylum level,the relative abundance of Bacteroidota,Desulfobacterota in CPFA group was lower than that in HC group,and the relative abundance of Firmicutes,Actinobacteria and Proteobacteria in CPFA group was higher than that in HC Group(P<0.05);③ At the genus level,the relative abundance of Bacteroids,Parabacteroides in the CPFA group was lower than that in the HC group,and the relative abundance of Bifidobacterium,Escherichia-Shigella and Veillonella in the CPFA group was higher than that in the HC group;④ Functional analysis showed that there were significant differences in functional genes between the two groups.Compared with those in the HC group,the abundance of functional genes in valine,leucine and isoleucine biosynthesis,cysteine and methionine metabolism,fatty acid metabolism and Staphylococcus aureus infection were significantly increased in the CPFA group(P<0.05).The abundance of functional pathways related to bile acid biosynthesis and retinol metabolism was significantly decreased(P<0.05).[Conclusion]There are differences in intestinal flora and function between children with CPFA and HC,which may help to explore the pathogenesis of food allergy and constipation,and provide a theoretical basis for new therapeutic interventions.关键词
食物过敏/便秘/肠道菌群/菌群功能/高通量测序Key words
food allergy/constipation/intestinal flora/microbiota function/high-throughput sequencing分类
医药卫生引用本文复制引用
吴慧,熊升华,黄从付..食物过敏伴便秘儿童肠道菌群变化与功能分析[J].中山大学学报（医学科学版）,2025,46(2):345-353,9.基金项目
深圳市龙岗区科技创新局基金(LGWJ2023-038) （LGWJ2023-038）
深圳市龙岗区妇幼保健院院级课题科研启动基金(Y2024011) （Y2024011）
