安徽农业科学2025，Vol.53Issue(7)：132-137,6.DOI:10.3969/j.issn.0517-6611.2025.07.023
多花黄精叶斑病病原鉴定及拮抗菌对其抑制作用研究
Identification of Pathogens of Polygonatum multiflorum Leaf Spot Disease and Their Inhibition by Antagonistic Bacteria
摘要
Abstract
Using plants with typical symptoms of Polygonatum multiflorum leaf spot disease as raw materials,the pathogenic fungi were isola-ted from the diseased leaves using tissue isolation method.Morphological observation and sequence analysis of ribosomal rDNA-ITS(internal transcribed spacer)region were used to classify and identify the pathogenic fungi.The inhibitory effects of three antagonistic bacteria:Glu-conobacter nephelii H-1,Bacillus subtilis H-3 and Bacillus magmatium B-2 on dominant pathogenic fungi were studied.The results showed that the main pathogens causing the leaf spot disease of Polygonatum multiflorum were Epicocum sorghum and Pestalotiopsis microspora;further re-search has found that the antagonistic bacterium of Bacillus subtilis H-3 had a strong inhibitory effect on both strains of pathogens,with inhibi-tion rates of 91.37%and 98.21%,respectively;Bacillus magnetrium B-2 also had a strong inhibitory effect with inhibition rates of 44.98%and 95.49%,respectively;while Gluconobacter nephelii H-1 on the two pathogenic fungi mentioned above was relatively weak,with inhibition rates of 31.89%and 50.41%,respectively.In summary,Bacillus subtilis has the best antibacterial effect and can provide a basis for the re-search and development of biopesticides in the later stage.关键词
多花黄精/叶斑病/病原菌/分离鉴定/拮抗菌/抑制作用Key words
Polygonatum multiflorum/Leaf spot disease/Pathogenic fungi/Separation and identification/Antagonistic bacteria/Inhibitory effect分类
植物保护学引用本文复制引用
胡秀虹,何佳,张廷辉,刘天宇,杨明豪..多花黄精叶斑病病原鉴定及拮抗菌对其抑制作用研究[J].安徽农业科学,2025,53(7):132-137,6.基金项目
2022年国家级大学生创新创业训练计划项目(202210669030) （202210669030）
2023年凯里学院专项课题(2023XJZX0304) （2023XJZX0304）
2022年度黔东南州基础研究计划项目(黔东南科合基础[2022]05号) （黔东南科合基础[2022]05号）
2022年省级"金课"(一流课程)建设项目(SJJK202221). （一流课程）
