安徽农业科学2025，Vol.53Issue(7)：146-149,159,5.DOI:10.3969/j.issn.0517-6611.2025.07.026
乌梅对柱状黄杆菌抑菌效果研究
Study on the Bacteriostatic Effect of Fructus mume on Flavobacterium columnare
摘要
Abstract
[Objective]To investigate the antibacterial effect of Fructus mume on Flavobacterium columnare.[Method]The antibacterial effect was determined by boiling Fructus mume to 100 mg/mL,adding the dosage of 1∶8,1∶16,1∶32,1∶64,1∶128 and 1∶256 into liquid medi-um,inoculating with F.columnare,observing and measuring absorbance after 72 h of culture.The pH of the liquid culture medium of Fructus mume with different addition ratios was determined,and the bacteriostatic test after pH to 7.2 adjustment was carried out.Then the concentra-tion of 0.2,0.4 and 0.6 mg/mL were added into solid medium to carry out plate inhibition test.[Result]The minimum inhibitory addition ra-tio of unadjusted pH liquid drug culture medium was 1∶128,which means the minimum inhibitory concentration was 0.775 mg/mL.When the addition ratio of Fructus mume was higher than 1∶128,it had a significant impact on pH,and the effect of pH changed on antibacterial activity was relatively small at lower concentrations;when the concentration of Fructus mume was 0.6 mg/mL,it could completely inhibit the growth of F.columnare.[Conclusion]Fructus mume has a good antibacterial effect on F.columnare;Fructus mume can change the pH of the culture medium,but its effect on the antibacterial test results is relatively small at lower doses;the minimum inhibitory concentration of Fructus mume against F.columnare is 0.6 mg/mL.关键词
乌梅/柱状黄杆菌/抑菌效果Key words
Fructus mume/Flavobacterium cloumnare/Bacteriostatic effect分类
中医学引用本文复制引用
张辽,周杰,欧阳高燚,刘万资..乌梅对柱状黄杆菌抑菌效果研究[J].安徽农业科学,2025,53(7):146-149,159,5.基金项目
河南省科技厅科技攻关项目(222102110374). （222102110374）
