南方医科大学学报2025，Vol.45Issue(4)：702-710,9.DOI:10.12122/j.issn.1673-4254.2025.04.04
生命早期两歧双歧杆菌BD-1干预可缓解注意缺陷多动障碍雌性大鼠幼年期的多动行为
Early life Bifidobacterium bifidum BD-1 intervention alleviates hyperactivity of juvenile female rats with attention deficit hyperactivity disorder
摘要
Abstract
Objective To investigate the effects of early life intervention with Bifidobacterium bifidum BD-1(B.bifidum BD-1)on hyperactivity in a female mouse model of attention deficit hyperactivity disorder(ADHD)and explore the underlying mechanisms.Methods Eight newborn female Wistar-Kyoto(WKY)rats and 6 spontaneous hypertensive rats(SHRs)were gavaged with saline and another 6 SHRs were gavaged with B.bifidum BD-1(109 CFU)daily for 3 weeks.Open field test of the rats was conducted at 7 weeks,and fecal samples were collected at weaning(3 weeks)and at 7 weeks for 16S rRNA sequencing.Immunofluorescent staining was used to detect dopamine transporter(DAT)and tyrosine hydroxylase(Th)levels in the striatum and activated microglia in the prefrontal cortex.Treg cells in the mesenteric lymph nodes,spleen and blood were analyzed using flow cytometry.Results The SHRs traveled a significantly greater distance in open fields test than WKY rats,and this behavior was significantly attenuated by B.bifidum BD-1 intervention.The expression of DAT and Th in the striatum was significantly lower in the SHRs than in WKY rats,while B.bifidum BD-1 treatment obviously increased Th levels in the SHRs.B.bifidum BD-1 intervention significantly deceased the number of activated microglia and increased Treg cell counts in the spleen of SHRs.The treatment also enhanced α diversity in gut microbiota of the SHRs and resulted in a decreased Firmicutes/Bacteroidota ratio,more active Muribaculaceae growth,and suppression of Clostridia_UCG-014 proliferation.Conclusion Early life intervention with B.bifidum BD-1 alleviates hyperactivity in female SHRs by modulating the gut microbiota and peripheral immune response,suppressing neuroinflammation and improving dopaminergic system function.These findings provide evidence for early prevention strategies and support the development and application of psychobiotics for ADHD.关键词
注意缺陷多动障碍/两歧双歧杆菌BD-1/微生物-肠-脑轴/生命早期/神经炎症Key words
attention deficit hyperactivity disorder/Bifidobacterium bifidum BD-1/microbiota-gut-brain axis/early life/neuroinflammation引用本文复制引用
杨洋,王凯,柳鉴修,周志谟,贾雯,吴思谋,李金星,何方,程如越..生命早期两歧双歧杆菌BD-1干预可缓解注意缺陷多动障碍雌性大鼠幼年期的多动行为[J].南方医科大学学报,2025,45(4):702-710,9.基金项目
国家自然科学基金青年科学基金(82204037) （82204037）
中国博士后科学基金(2022M712228) （2022M712228）
四川大学专职博士后科研基金(2022SCU12026) Supported by Youth Science Foundation of National Natural Science Foundation of China(82204037). （2022SCU12026）
