南方医科大学学报2025，Vol.45Issue(4)：744-750,7.DOI:10.12122/j.issn.1673-4254.2025.04.09
大麻二酚通过调节昼夜节律改善大鼠全麻术后的睡眠障碍
Cannabidiol regulates circadian rhythm to improve sleep disorders following general anesthesia in rats
摘要
Abstract
Objective To assess the regulatory effect of cannabidiol(CBD)on circadian rhythm sleep disorders following general anesthesia and explore its potential mechanism in a rat model of propofol-induced rhythm sleep disorder.Methods An electrode was embedded in the skull for cortical EEG recording in 24 male SD rats,which were randomized into control,propofol,CBD treatment,and diazepam treatment groups(n=6).Eight days later,a single dose of propofol(10 mg/kg)was injected via the tail vein with anesthesia maintenance for 3 h in the latter 3 groups,and daily treatment with saline,CBD or diazepam was administered via gavage;the control rats received only saline injection.A wireless system was used for collecting EEG,EMG,and body temperature data within 72 h after propofol injection.After data collection,blood samples and hypothalamic tissue samples were collected for determining serum levels of oxidative stress markers and hypothalamic expressions of the key clock proteins.Results Compared with the control rats,the rats with CBD treatment showed significantly increased sleep time at night(20:00-6:00),especially during the time period of 4:00-6:00 am.Compared with the rats in propofol group,which had prolonged SWS time and increased sleep episodes during 18:00-24:00 and sleep-wake transitions,the CBD-treated rats exhibited a significant reduction of SWS time and fewer SWS-to-active-awake transitions with increased SWS aspects and sleep-wake transitions at night(24:00-08:00).Diazepam treatment produced similar effect to CBD but with a weaker effect on sleep-wake transitions.Propofol caused significant changes in protein expressions and redox state,which were effectively reversed by CBD treatment.Conclusion CBD can improve sleep structure and circadian rhythm in rats with propofol-induced sleep disorder possibly by regulating hypothalamic expressions of the key circadian clock proteins,suggesting a new treatment option for perioperative sleep disorders.关键词
术后睡眠障碍/丙泊酚/大麻二酚/时钟蛋白Key words
postoperative sleep disturbance/propofol/cannabidiol/circadian clock proteins引用本文复制引用
吴新顺,董华进,张有志,曹江北,李劲草,刘影,邱仁洪,王恒林,薛瑞,张扬,李硕,范琼尹..大麻二酚通过调节昼夜节律改善大鼠全麻术后的睡眠障碍[J].南方医科大学学报,2025,45(4):744-750,7.基金项目
国家自然科学基金(82271213) （82271213）
国家重点新药开发计划(2017ZX09309020) Supported by National Natural Science Foundation of China(82271213). （2017ZX09309020）
