南方医科大学学报2025，Vol.45Issue(4)：751-759,9.DOI:10.12122/j.issn.1673-4254.2025.04.10
激活下丘脑背内侧核区星形胶质细胞可加速七氟醚麻醉小鼠觉醒
Activation of astrocytes in the dorsomedial hypothalamus accelerates sevoflurane anesthesia emergence in mice
摘要
Abstract
Objective To investigate the regulatory role of astrocytes in the dorsomedial hypothalamus(DMH)during sevoflurane anesthesia emergence.Methods Forty-two male C57BL/6 mice were randomized into 6 groups(n=7)for assessing astrocyte activation in the dorsomedial hypothalamus(DMH)under sevoflurane anesthesia.Two groups of mice received microinjection of agfaABC1D promoter-driven AAV2 vector into the DMH for GCaMP6 overexpression,and the changes in astrocyte activity during sevoflurane or air inhalation were recorded using calcium imaging.For assessing optogenetic activation of astrocytes,another two groups of mice received microinjection of an optogenetic virus or a control vector into the DMH with optic fiber implantation,and sevoflurane anesthesia emergence was compared using behavioral experiments.In the remaining two groups,electroencephalogram(EEG)recording during sevoflurane anesthesia emergence was conducted after injection of the hChR2-expressing and control vectors.Anesthesia induction and recovery were assessed by observing the righting reflex.EEG data were recorded under 2.0%sevoflurane to calculate the burst suppression ratio(BSR)and under 1.5%sevoflurane for power spectrum analysis.Immunofluorescence staining was performed to visualize the colocalization of GFAP-positive astrocytes with viral protein signals.Results Astrocyte activity in the DMH decreased progressively as sevoflurane concentration increased.During 2.0%sevoflurane anesthesia,the mice injected with the ChR2-expressing virus exhibited a significantly shortened wake-up time(P<0.05),and optogenetic activation of the DMH astrocytes led to a marked reduction in BSR(P<0.001).Under 1.5%sevoflurane anesthesia,optogenetic activation resulted in a significant increase in EEG gamma power and a significant decrease in delta power in ChR2 group(P<0.01).Conclusion Optogenetic activation of DMH astrocytes facilitates sevoflurane anesthesia emergence but does not significantly influence anesthesia induction.These findings offer new insights into the mechanisms underlying anesthesia emergence and may provide a potential target for accelerating postoperative recovery and managing anesthesia-related complications.关键词
星形胶质细胞/下丘脑背内侧核区/七氟烷/麻醉觉醒Key words
astrocytes/dmh region/sevoflurane/anesthesia emergence引用本文复制引用
郭舒婷,曹福羊,郭永馨,李言响,郝新宇,张倬宁,周志康,仝黎,曹江北..激活下丘脑背内侧核区星形胶质细胞可加速七氟醚麻醉小鼠觉醒[J].南方医科大学学报,2025,45(4):751-759,9.基金项目
国家自然科学基金(82271213) Supported by National Natural Science Foundation of China(82271213). （82271213）
