南方医科大学学报2025，Vol.45Issue(4)：862-870,9.DOI:10.12122/j.issn.1673-4254.2025.04.22
类风湿性关节炎中氧化应激与免疫浸润的生物信息学分析
Bioinformatics analysis of oxidative stress and immune infiltration in rheumatoid arthritis
摘要
Abstract
Objective To explore the role of oxidative stress and immune infiltration in rheumatoid arthritis(RA).Methods RA datasets GSE55235(10 RA vs 10 normal samples)and GSE55457(13 RA vs 10 normal samples)from the GEO database were merged as the test set to identify the differentially expressed genes(DEGs)in RA using R.The DEGs were intersected with oxidative stress-related genes to obtain oxidative stress-associated DEGs.KEGG and GO enrichment analyses of the DEGs were performed,and the RA-related pathways and biological processes were analyzed using GSEA.A protein-protein interaction(PPI)network was constructed using STRING and Cytoscape,and the top 10 key genes were obtained using the Degree algorithm.The validation dataset GSE1919 from GEO database was used for ROC analysis of the key genes to obtain the core genes,and their correlations with infiltrating immune cells were analyzed using CIBERSORT.The results were verified by RT-qPCR for detecting expression levels of the core genes in RA and normal joint samples.Results We identified 89 oxidative stress-associated DEGs.Enrichment analysis suggested that these DEGs were involved in the biological processes including oxidative stress,chemical stress response,reactive oxygen species response,and lipopolysaccharide response.ROC analysis showed that the 5 core genes(STAT1,MMP9,MYC,CCL5,and JUN)all had AUC values>0.7,indicating their high diagnostic sensitivity and specificity for RA.These genes were closely correlated with immune cells,particularly T cells.RT-qPCR confirmed significant differential expressions of the core genes between RA and normal samples.Conclusion Oxidative stress and diverse immune responses are features of RA,and the immune responses contribute to activation of oxidative stress.The identified core genes can potential serve as new diagnostic markers for RA.关键词
类风湿关节炎/氧化应激/免疫浸润/生物标志物/生物信息学Key words
rheumatoid arthritis/oxidative stress/immune infiltration/biomarkers/bioinformatics引用本文复制引用
高志,吴傲,胡仲翔,孙培养..类风湿性关节炎中氧化应激与免疫浸润的生物信息学分析[J].南方医科大学学报,2025,45(4):862-870,9.基金项目
第五批全国中医临床优秀人才研修项目(国中医药人教函[2022]1号) （国中医药人教函[2022]1号）
第七批全国老中医药专家学术经验继承工作项目(国中医药人教函[2022]76号) （国中医药人教函[2022]76号）
安徽省第一届卫生健康杰出人才项目(皖卫函[2022]392号) （皖卫函[2022]392号）
储浩然全国名老中医药专家传承工作室(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
安徽省重大疑难疾病中西医协同攻关项目(皖中医药发展秘[2021]70号) （皖中医药发展秘[2021]70号）
黄学勇安徽省名中医工作室(中发展[2022]5号) （中发展[2022]5号）
国家中医优势专科(脑病科)建设项目(国中医药医政函[2024]90号) （脑病科）
