森林与环境学报2025，Vol.45Issue(3)：273-282,10.DOI:10.13324/j.cnki.jfcf.202412014
红松种源遗传多样性评价和育种群体构建
Genetic diversity evaluation and breeding population construction of Pinus koraiensis provenance
摘要
Abstract
Pinus koraiensis is one of the most important and valuable tree species in northeast China.To meet the needs of evaluation,excavation,and utilization of Pinus koraiensis germplasm resources,this study employed morphological and molecular methods to investigate the genetic diversity and construct a breeding population of Pinus koraiensis from 12 different provenances in Liaoning,Jilin,and Heilongjiang.The results are expected to reveal the morphological and molecular genetic differentiation of this plant.The results showed that the genetic variations were mainly within provenances,with morphological and molecular variations accounting for 67.36%and 94.00%of the total variation,respectively.The coefficients of variation of various growth traits among different provenances ranged from 14.77%to 28.55%.The overall genetic diversity was high,with a Shannon index of 0.665.Yichun provenance(No.31)had high genetic diversity,with the diversity index and Shannon index being 1.65 and 0.739,respectively.A breeding population was constructed based on molecular and phenotypic markers,consisting of 38 plants.The effective allele(Ne),Shannon index,and observed heterozygosity(Ho)of this population were 1.989,0.921,and 0.921,respectively,which demonstrated that the population had certain representativeness.关键词
红松/生长性状/简单重复序列/遗传多样性/育种群体Key words
Pinus koraiensis/growth traits/simple sequence repeat(SSR)/genetic diversity/breeding population分类
林学引用本文复制引用
尚福强,马晓雨,高源,张利民,张丽艳..红松种源遗传多样性评价和育种群体构建[J].森林与环境学报,2025,45(3):273-282,10.基金项目
辽宁省重点研发计划项目"红松优良种质资源收集与利用研究"(2020JH2/10200020) （2020JH2/10200020）
辽宁省农业科学院学科绩效后补助项目"基于种实性状和分子标记的红松群体遗传多样性研究"(2023-HBZ-1010) （2023-HBZ-1010）
辽宁省农业科学院院长基金项目"基于SSR标记的红松种子园无性系遗传多样性分析及分子身份证构建"(2024QN1817). （2024QN1817）
