康复学报2025，Vol.35Issue(2)：160-168,9.DOI:10.3724/SP.J.1329.2025.02008
督脉电针联合跑台训练对脊髓损伤大鼠运动功能的影响
Effects of Du Meridian Electroacupuncture Combined with Treadmill Training on Motor Function of Rats with Spinal Cord Injury
摘要
Abstract
Objective To explore the effects and underlying mechanism of Du meridian electroacupuncture combined with treadmill training on motor function of rats with spinal cord injury(SCI).Methods A total of 50 healthy female SPF-grade SD rats were randomly divided into sham operation group,model group,treadmill group,electroacupuncture group and treadmill+elec-troacupuncture group,with 10 rats in each group.In the sham group,the T10 laminectomy was performed without SCI;while in the other four groups,Allen's method and New York University(NYU)spinal cord injury impactor method were used to establish T10 SCI model.Rats with death or BBB motor function scale score>2 were excluded,and finally eight rats were included in each group.The sham operation group and the model group had free diet and activities without any intervention.The treadmill group received treadmill training,once a day,30 minutes a time,five days a week for four weeks.The electroacupuncture group received electroacu-puncture stimulation at Dazhui(DU14)and Mingmen(GV4),once a day,20 minutes a time,five times a week for four weeks.The treadmill+electroacupuncture group received treadmill training followed by electroacupuncture stimulation,and the training methods were the same as those of the treadmill group and the electroacupuncture group.Before and after the operation,BBB scale was used to evaluate the recovery of motor function after SCI.After operation,magnetic resonance imaging(MRI)was used to detect the re-covery of the SCI segment;immunohistochemistry was used to detect the protein expression levels of brain-derived neurotrophic fac-tor(BDNF)and nerve growth factor(NGF);Western blot was used to detect the protein expression levels of postsynaptic density-95(PSD-95)and SynapsinⅠ.Results(1)BBB score:compared with the model group at the same time,BBB scores of the treadmill group,the electroacupuncture group and the treadmill+electroacupuncture group increased significantly at 2,3,4 weeks after opera-tion,and the differences were statistically significant(P<0.05).Compared with the treadmill group and the electroacupuncture group at the same time,BBB score of the treadmill+electroacupuncture group increased significantly at 3,4 weeks after operation,and the differences were statistically significant(P<0.05).(2)Recovery of the SCI segment:compared with the model group,the spinal cord tissues in the treadmill group and the electroacupuncture group recovered significantly,and the inflammatory reaction area was re-duced.Compared with the model group,the treadmill group and the electroacupuncture group,the treadmill+electroacupuncture group showed more complete spinal cord structure,smaller injury range,lower MRI hyperintensity,and less inflammatory reaction.(3)Protein expression levels of BDNF and NGF:compared with the sham operation group,the model group,the treadmill group and the electroacupuncture group,the protein expression level of BDNF in the treadmill+electroacupuncture group was significantly higher(P<0.05).Compared with the model group,the protein expression level of NGF in the treadmill+electroacupuncture group in-creased significantly(P<0.05).(4)Protein expression levels of PSD-95 and SynapsinⅠ:compared with the sham operation group,the protein expression levels of PSD-95 and SynapsinⅠ in the model group and the electroacupuncture group decreased significantly(P<0.05),the protein expression levels of PSD-95 and SynapsinⅠ in the treadmill group and the treadmill+electroacupuncture group increased significantly(P<0.05).Compared with the model group,the protein expression levels of PSD-95 and SynapsinⅠ in the treadmill group and the treadmill+electroacupuncture group increased significantly(P<0.05),and the protein expression level of PSD-95 in the electroacupuncture group decreased significantly(P<0.05),while the protein expression level of SynapsinⅠ increased significantly(P<0.05).Compared with the treadmill group,the protein expression levels of PSD-95 and SynapsinⅠ in the elec-troacupuncture group decreased significantly(P<0.05),and the protein expression levels of PSD-95 and SynapsinⅠ in the treadmill+electroacupuncture group increased significantly(P<0.05).Compared with the electroacupuncture group,the protein expression levels of PSD-95 and SynapsinⅠ in the treadmill+electroacupuncture group increased significantly(P<0.05).Conclusion Du meridian electroacupuncture combined with treadmill training can effectively improve the spinal cord injury and motor function of SCI rats,possibly by regulating the protein expression levels of BDNF,NGF,PSD-95 and SynapsinⅠ.关键词
脊髓损伤/督脉/电针/跑台训练/运动功能/大椎穴(DU14)/命门穴(GV4)Key words
spinal cord injury/Du meridian/electroacupuncture/treadmill training/motor function/Dazhui acupoint(DU14)/Mingmen acupoint(GV4)引用本文复制引用
程枚,田宇,彭京,余双奇,张学俊,丁凡..督脉电针联合跑台训练对脊髓损伤大鼠运动功能的影响[J].康复学报,2025,35(2):160-168,9.基金项目
国家自然科学基金青年科学基金项目(81601934) （81601934）
湖北省卫生健康科研基金项目(WJ2021F015) （WJ2021F015）
