辽宁石油化工大学学报2025，Vol.45Issue(2)：53-60,8.DOI:10.12422/j.issn.1672-6952.2025.02.007
Brucella Intermedi降解条件优化及其对原油的防蜡效果分析
Optimization of Growth and Degradation Conditions of Brucella Intermedia and Analysis of Its Wax Control Effect on Crude Oil
摘要
Abstract
During the extraction and transportation of waxy crude oil,paraffin will deposit on the wall,forming wax deposition.In recent years,microbial wax removal and prevention technology has been widely studied for its economic and environmental advantages.Five strains of bacteria were screened from crude oil sludge,and through the determination of their paraffin degradation rate and surface hydrophobicity,the bacterium B3 was selected and identified as Bruella intermedia.The experimental results showed that bacterial B3 had the best growth activity at a temperature of 40℃,an initial pH of 6,and a shaking table speed of 160 r/min,and had the best degradation effect on paraffin at this time.When bacteria B3 grows and metabolizes with paraffin as a carbon source,they can produce lipopeptide biosurfactants,with an emulsification coefficient of 52.5%for liquid paraffin.After 7 days of interaction between bacteria B3 and crude oil,the wax prevention rate reached 77.2%,and the viscosity reduction rate reached 50.2%at 41℃.Bacterial B3 can degrade paraffin,improve crude oil fluidity,and reduce wax deposition.关键词
中间布鲁氏菌/生物表面活性剂/防蜡率/降黏Key words
Brucella intermedia/Biosurfactants/Anti wax effect/Reducing viscosity分类
石油、天然气工程引用本文复制引用
王慧,张海娟,王卫强,杨立鉴..Brucella Intermedi降解条件优化及其对原油的防蜡效果分析[J].辽宁石油化工大学学报,2025,45(2):53-60,8.基金项目
辽宁省教育厅项目(L2020027) （L2020027）
抚顺英才计划项目(FSYC202101001). （FSYC202101001）
