辐射研究与辐射工艺学报2025，Vol.43Issue(2)：72-85,14.DOI:10.11889/j.1000-3436.2024-0088
辐照-水热处理制备油茶壳低聚木糖的脱色工艺与抗氧化性研究
Study on the decolorization process and antioxidant activity of xylo-oligosaccharides in Camellia oleifera shell prepared via irradiation-hydrothermal treatment
摘要
Abstract
Xylo-oligosaccharides were prepared from Camellia oleifera shell via hydrothermal treatment after electron beam irradiation.The antioxidant activity of xylo-oligosaccharide of Camellia oleifera shell after decolorization was determined and analyzed through optimizing the decolorization process of activated carbon.This provides a new approach for the high-value utilization of by-products from Camellia oleifera processing.The results showed that the concentration of xylo-oligosaccharides prepared via 3 MeV electron beam irradiation combined with hydrothermal treatment was 13.87 mg/mL,and the concentrations of xylobiose to xylotetrose after enzymolysis were 3.37 mg/mL,4.76 mg/mL,and 2.48 mg/mL,respectively.The optimization process of activated carbon decolorization as follows:the decolorization efficiency of Darco G60-activated carbon for xylo-oligosaccharides enzymolysis solution of the Camellia oleifera shell reached 93.77%at a decolorization temperature of 30℃,a decolorization time of 60 min,an activated carbon addition of 50 mg/mL,and a pH of 4.The retention rates of xylobiose to xylotetrose and total xylo-oligosaccharides were 97.66%and 71.50%,respectively.It was found that the xylo-oligosaccharides of the Camellia oleifera shell were amorphous and cross-linked with several parts,as shown by scanning electron microscopy and Fourier transform infrared spectroscopy,with the main chain formed by D-xylose via β-1,4-glucoside linkage.In addition,when the concentration of xylo-oligosaccharides in Camellia oleifera shell was 10 mg/mL,the antioxidant activity was better.The scavenging rates of 1,1-Diphenyl-2-picrylhydrazyl(DPPH)radical,2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radical,superoxide anion and hydroxyl radical were 62.07%,68.50%,70.26%and 75.52%,respectively.关键词
辐照-水热处理/低聚木糖/活性炭/结构分析/抗氧化Key words
Irradiation-hydrothermal treatment/Xylo-oligosaccharides/Activated carbon/Structural analysis/Antioxidant分类
轻工纺织引用本文复制引用
沈晓岩,陈亮,齐慧,刘安,魏东宁,邓明,王克勤,武小芬..辐照-水热处理制备油茶壳低聚木糖的脱色工艺与抗氧化性研究[J].辐射研究与辐射工艺学报,2025,43(2):72-85,14.基金项目
国家自然科学基金(12205094)、湖南省重点研发项目(2023NK2040-3)、湖南省农业科技创新资金(2023CX59)资助 Supported by National Natural Science Foundation of China(12205094),the Hunan Provincial Key Research and Development Projects(2023NK2040-3),and the Agricultural Science and Technology Innovation Funding Program of the Hunan Province(2023CX59) （12205094）
