广东农业科学2025，Vol.52Issue(2)：58-70,13.DOI:10.16768/j.issn.1004-874X.2025.02.005
一株猪源肠出血性大肠杆菌O157∶H7的全基因组测序及毒力和耐药基因分析
Whole Genome Sequencing Reveals the Distribution of Resistance and Virulence Genes of an Enterohemorrhagic Escherichia coli O157∶H7 Strain from Swine
摘要
Abstract
[Objective]In order to analyze the whole genome information of a porcine derived enterohemorrhagic Escherichia coli O157∶H7 strain EO157-1 and its virulence and drug resistance genes.[Method]This experiment conducted whole-genome sequencing of enterohemorrhagic E.coli O157∶H7 strain EO157-1 based on the Nanopore third-generation sequencing technology platform and the Illumina second-generation sequencing technology platform.The Prokka software was used to predict the genome sequence information,and the genome sequence information was predicted through UniProt,KEGG and KEGG Pathway,GO,Pfam,COG,TIGERfams,RefSeq and NR databases were used for gene prediction and annotation,and then the carbohydrate enzyme(CAZy),pathogen and host interaction(PHI),virulence factor(VFDB)and drug resistance gene(CARD)databases were used Perform gene functional analysis.[Result]The genome size of enterohemorrhagic E.coli O157∶H7 strain EO157-1 is 5 404 747 bp,and the GC content is 50.49%.In addition,this strain also contains 3 plasmids.There are 5 674 predicted protein-coding genes,including 22 rRNA(7 23S rRNA,7 16S rRNA,8 5S rRNA),1 tmRNA,102 tRNA genes and 282 small RNA molecules;9 are predicted CRISPR sequences,273 repeat sequences;KEGG,KEGG Pathway,NR,UniProt,GO,COG,Pfam,RefSeq and TIGERfams databases annotated 1 712,1 693,5 265,5 264,4 029,4 294,4 688,5 252 and 2 741 genes respectively.The CAZy database annotation screened out 100 genes,the PHI database annotation screened out 2 354 genes,and the VFDB database annotation screened out virulence genes mainly including pili,flagella,type II secretion system,type III secretion system,type VI secretion system,intimin,iron transport system,lipopolysaccharide,capsule,invasins and exotoxins;resistance genes and multidrug resistance efflux pumps,macrolide antibiotics,tetracyclines,polymyxins,bacitracin,and aminoglycoside antibiotics,peptide antibiotics and metronidazole related.[Conclusion]The whole genome sequence of an enterohemorrhagic E.coli O157∶H7 strain EO157-1 was obtained.Sequence analysis showed that this strain carries a large number of virulence genes and drug resistance genes.关键词
肠出血性大肠杆菌O157∶H7/食源性人兽共患病/全基因组测序/基因/功能注释Key words
enterohemorrhagic Escherichia coli O157:H7/foodborne zoonoses/whole genome sequencing/genes/functional annotation分类
农业科技引用本文复制引用
周艳,万启旸,朱树娇,张辉,包红朵,王冉..一株猪源肠出血性大肠杆菌O157∶H7的全基因组测序及毒力和耐药基因分析[J].广东农业科学,2025,52(2):58-70,13.基金项目
国家自然科学基金(31972764) （31972764）
