广东农业科学2025，Vol.52Issue(2)：117-125,9.DOI:10.16768/j.issn.1004-874X.2025.02.010
浙江省葡萄炭疽病菌的分离鉴定及生物学特性研究
Isolation,Identification and Biological Characteristics of Grape Anthracnose Pathogen in Zhejiang Province
摘要
Abstract
[Objective]Grape anthracnose is one of the most serious and common grape diseases in China.The pathogens involve multiple species and subspecies of Colletotrichum gloeosporioides and C.acutatum complex.The growth and epidemic characteristics of different species of pathogens are significantly different.Clarifying the species of grape anthracnose pathogens in Zhejiang Province can provide a reference for the population distribution of grape anthracnose pathogens and the green prevention and control of diseases in China.[Method]Grape anthracnose samples were collected,and pathogens were isolated from the diseased parts of fruits.The pathogen was purified by subculture,and the colony,spore morphology and appressorium morphology of the pathogen were observed.The phylogenetic tree was constructed by using the sequences of ITS,TUB2,GAPDH,ACT,CHS and CAL.The pathogenicity of the pathogen was determined by artificial inoculation,and the effects of different temperature,pH value,carbon source and nitrogen source on the growth of the pathogen were determined.[Result]Six strains of pathogens with basically the same morphological characteristics were isolated from the diseased parts of grape fruits.Koch's test showed that the strain was the pathogen of the disease.Combined with the morphological characteristics of the pathogen and the phylogenetic analysis based on ITS,TUB2,GAPDH,ACT,CHS and CAL gene sequences,the pathogen was identified as C.aenigma.The biological characteristics of the pathogen were analyzed.The results showed that the optimum culture temperature and sporulation temperature of the pathogen were 28℃,the optimum growth pH was 6,and the optimum carbon and nitrogen sources were maltose and beef extract,respectively.[Conclusion]In recent years,the pathogen of grape anthracnose in Ninghai County,Zhejiang Province is mainly C.aenigma.And its biological characteristis were clarified.关键词
葡萄炭疽病/隐秘刺盘孢菌/组织分离/病原菌鉴定/系统发育树/生物学特性Key words
grapevine anthracnose/Colletotrichum aenigma/tissue separation/pathogen identification/phylogenetic tree/biological characterization分类
农业科技引用本文复制引用
虞沈丹,路子琪,柴荣耀,武军,王静,邱海萍,王艳丽,孙国仓,王教瑜..浙江省葡萄炭疽病菌的分离鉴定及生物学特性研究[J].广东农业科学,2025,52(2):117-125,9.基金项目
浙江省"尖兵·领雁"重点研发计划项目(2023C02018) （2023C02018）
国家现代农业产业技术体系建设专项(CARS-29-13) （CARS-29-13）
