广西医科大学学报2025，Vol.42Issue(2)：168-173,6.DOI:10.16190/j.cnki.45-1211/r.2025.02.002
果糖通过促进糖酵解调控M1型巨噬细胞极化
Fructose regulates M1 macrophage polarisation by promoting glycolysis
摘要
Abstract
Objective:To investigate the regulatory effect of fructose(Fru)on the polarization of M1 macro-phages induced by lipopolysaccharide(LPS)and interferon gamma(IFN-γ)and its possible mechanism.Meth-ods:The experiment was divided into M0 group(THP-1 cells were stimulated with 100 nmol/L phorbol esters for 24 h),M1 group(M0 group was stimulated with 10 ng/mL LPS and 20 ng/mL IFN-γ for 48 h),and M1+5 mmol/L Fru group(5 mmol/L Fru was added simultaneously with LPS and IFN-γ for 48 h of stimulation).Reverse transcription-quantitative polymerase chain reaction(RT-qPCR)was used to detect the mRNA expression of po-larization markers interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and cyclooxygen-ase-2(COX-2)in M1 macrophages.The activities of glycolytic rate-limiting enzyme hexokinase(HK)and lactate dehydrogenase(LDH)were detected using a microenzyme activity kit.The reactive oxygen species(ROS)kits were used to detect intracellular ROS levels.The phagocytic ability of the cells was detected by neutral erythro-phagocytosis assay.The mitochondrial respiratory chain metabolic enzyme activities were detected by Azulin method.The adenosine triPhosphate(ATP)contents were detected by using an ATP test kit.Results:Compared with the M0 group,mRNA expression of IL-1β,IL-6,TNF-α and COX-2 in the M1 group was increased,activi-ties of HK and LDH were increased,mitochondrial respiratory chain metabolic enzyme activities and ATP con-tents were decreased,intracellular ROS production was increased,and phagocytic ability was enhanced(all P＜0.05).Compared with the M1 group,mRNA expression levels of IL-1β,IL-6,TNF-α and COX-2 in the M1+5 mmol/L Fru group were increased,activities of HK and LDH were increased,mitochondrial respiratory chain metabolic enzyme activities and ATP contents were decreased,intracellular ROS production was increased,and phagocytic ability was decreased(all P＜0.05).Conclusion:Fructose may promote the glycolysis of M1 macro-phages,reduce the level of oxidative phosphorylation,upregulate the expression of polarization related markers of M1 macrophages,promote the production of ROS,and downregulate the phagocytic ability of macrophages,thereby regulating the polarization of M1 macrophages.关键词
果糖/M1型巨噬细胞/糖酵解/极化/吞噬能力Key words
fructose/M1 macrophages/glycolysis/polarisation/phagocytic ability分类
预防医学引用本文复制引用
王名鸿,李习艺,刘彧冰,王新航,李菡,陈思宏,张敏华,彭阳,陆彩玲,唐深..果糖通过促进糖酵解调控M1型巨噬细胞极化[J].广西医科大学学报,2025,42(2):168-173,6.基金项目
国家自然科学基金资助项目(No.82460638 （）
No.82160612) （）
