广西医科大学学报2025，Vol.42Issue(2)：210-217,8.DOI:10.16190/j.cnki.45-1211/r.2025.02.007
桐花树土壤内生真菌Aspergillus fumigatus 06T03次生代谢产物的分离及其生物活性研究
Study on the isolation of secondary metabolites and their biological activities of the endo-phytic fungus Aspergillus fumigatus 06T03 from the soil of Aegiceras corniculatum
摘要
Abstract
Objective:To investigate the isolation and purification of the secondary metabolites and their poten-tial biological activity in the endophytic fungus Aspergillus fumigatus 06T03 from the soil of Aegiceras cornicula-tum.Methods:The strain was fermented on a large scale using rice medium,and then extracted by soaking ethyl acetate and concentrated under reduced pressure to obtain a crude extract of ethyl acetate.A variety of modern chromatographic techniques such as silica gel column chromatography,Sephadex LH-20 gel column chromatog-raphy,high-performance liquid chromatography were used to separate the chemical components of the crude ex-tract.Their structures were determined by using comprehensive spectral techniques such as high-resolution mass spectra(MS)and nuclear magnetic resonance(NMR)spectroscopy.The antineoplastic and anti-inflammatory ac-tivities of the monomers were evaluated using cell counting kit-8(CCK-8)assay and Griess assays.Results:Nine compounds were isolated from the rice fermentation products of Aspergillus fumigatus 06T03 and identified as Monomethylsulochrin(1),Questin(2),Demethylsulochrin(3),Fumiquinazoline C(4),Fumiquinazoline J(5),Azaspirofurans A(6),Pseurotin A(7),N-[2-(4-hydroxyphenyl)ethenyl]formamide(8),and Pyripyropene A(9).Among them,compounds 1-3 belonged to anthraquinones,and 4-9 belonged to alkaloids.The results showed that compounds 1-9 failed to exhibit obvious antineoplastic activity against Huh7 cells,ovarian carcinoma cells(A2780),and breast cancer cells(MDA-MB-231),while compounds 1,3,6,7,8,and 9 had certain anti-inflammatory activities.Conclusion:Nine monomeric compounds,except compound 2 which has been isolated before,have been isolated for the first time from the endophytic fungus Aspergillus fumigatus 06T03 from the soil of Aegiceras corniculatum.Compounds 1,3,6,7,8,and 9 have certain anti-inflammatory activities.This study enriches the chemical diversity of the secondary metabolites of endophytic fungus from the soil of Aegiceras cor-niculatum,and provides basic data for subsequent further development.关键词
桐花树/内生真菌/烟曲霉/次生代谢产物/生物活性Key words
Aegiceras corniculatum/endophytic fungus/Aspergillus fumigatus/secondary metabolites/biologi-cal activity分类
生物科学引用本文复制引用
代子腾,朱丹,韦霞,刘妍颖,游证喨,吴承华,王娟,沈张扬,幸尚平,黄倩婵..桐花树土壤内生真菌Aspergillus fumigatus 06T03次生代谢产物的分离及其生物活性研究[J].广西医科大学学报,2025,42(2):210-217,8.基金项目
国家自然科学基金资助项目(No.82160734) （No.82160734）
广西科技重大专项资助(No.桂科AA22362) （No.桂科AA22362）
中央引导地方科技发展资金资助项目(No.桂科ZY24212031) （No.桂科ZY24212031）
广西中医药多学科交叉创新团队资助项目(No.GZKJ2305) （No.GZKJ2305）
广西医科大学青年基金项目(No.GXMUYSF202315) （No.GXMUYSF202315）
大学生创新创业训练项目资助(No.202410598031) （No.202410598031）
