广西医科大学学报2025，Vol.42Issue(2)：226-232,7.DOI:10.16190/j.cnki.45-1211/r.2025.02.009
青蒿琥酯通过抑制Stat3/TGF-β1信号通路改善大鼠头颈放射性皮肤纤维化的实验研究
Experimental study of artesunate ameliorating head-and-neck radiation-induced skin fibrosis in rats through inhibiting Stat3/TGF-β1 signaling pathway
摘要
Abstract
Objective:To study the effect and mechanism of artesunate(ART)in improving radiation-induced skin fibrosis(RISF)induced by head-and-neck radiotherapy in rats.Methods:Thirty Sprague-Dawley rats were randomly divided into three groups:a control group,an irradiation only group(IR group),and an ART interven-tion group(ART+IR group),with 10 rats in each group.A rat model of head-and-neck radiotherapy was estab-lished by using medical linear electron accelerator with a single dose of 18 Gy.The ART+IR group was adminis-tered ART(20 mg/kg)intragastrically once a day,3 days prior to radiation and continued for 4 weeks.Twelve weeks after the model establishment,rats were sacrificed and tissue collected.Hematoxylin-eosin(HE)staining,Masson staining and immunohistochemical staining were used to observe the histopathological condition of the skin tissue in the irradiated area of the rats,the changes of the dermal thickness,and the expression of α-SMA pro-tein in the skin tissue;the mRNA expression of Stat3,TGF-β1,and interleukin-6(IL-6)was detected by reverse transcription-quantitative polymerase chain reaction(RT-qPCR);the western blotting was used to detect the pro-tein expression of p-Stat3,Stat3,TGF-β1,α-SMA,and IL-6.Results:ART intervention reduced skin appearance damage,pathological damage,improved dermal thickness and collagen deposition in head-and-neck radiotherapy rats(P＜0.05),and reduced α-SMA expression in skin tissue of rats after radiotherapy;it significantly down-regulated the protein and mRNA expression levels of p-Stat3/Stat3,TGF-β1,and IL-6(P＜0.05).Conclusion:ART can improve RISF after head-and-neck radiotherapy in rats,and its mechanism may be related to the down-regulation of Stat3/TGF-β1 signaling pathway and thus inhibit inflammation.关键词
放射性皮肤纤维化/青蒿琥酯/Stat3/TGF-β1Key words
radiation-induced skin fibrosis/artesunate/Stat3/TGF-β1分类
医药卫生引用本文复制引用
唐婵,欧剑波,农晓琳..青蒿琥酯通过抑制Stat3/TGF-β1信号通路改善大鼠头颈放射性皮肤纤维化的实验研究[J].广西医科大学学报,2025,42(2):226-232,7.基金项目
国家自然科学基金资助项目(No.81060156) （No.81060156）
广西自然科学基金面上项目资助(No.2024GXNSFAA010359) （No.2024GXNSFAA010359）
