广西医科大学学报2025，Vol.42Issue(2)：247-254,8.DOI:10.16190/j.cnki.45-1211/r.2025.02.012
沉香提取物通过调控NF-κB通路改善HaCaT细胞银屑病样炎症
Agarwood extract improves psoriatic-like inflammation in HaCaT cells by regulating NF-κB pathway
摘要
Abstract
Objective:To investigate the effect and mechanism of agarwood extract(AE)on tumor necrosis fac-tor-α(TNF-α)-induced psoriatic-like inflammation model of keratinocytes(HaCaT).Methods:The psoriatic-like inflammation model of HaCaT cells was induced by 40 ng/mL TNF-α,and then the cells were treated with AE at concentrations of 16 μg/mL(low concentration),24 μg/mL(medium concentration),32 μg/mL(high concentra-tion).Cell counting kit-8(CCK-8)assay was used to detect the viability of HaCaT cells.Flow cytometry was ap-plied to detect the apoptosis of HaCaT cells.Reverse transcription-quantitative polymerase chain reaction(RT-qPCR)and enzyme-linked immunosorbent assay(ELISA)were used to detect the mRNA expression and secre-tion levels of various inflammatory mediators,as well as that of inhibitor kappa B alpha(IκBα).Western blotting was used to detect the expression of key proteins in the NF-κB pathway.Results:After 40 ng/mL TNF-α stimula-tion,the viability of HaCaT cells was increased(P＜0.01),the early apoptosis rate and IκBα mRNA level were de-creased(P＜0.05),and the mRNA expression levels and release of interleukin-6(IL-6),interleukin-1β(IL-1β),interleukin17A(IL-17A),TNF-α,chemokine ligand 20(CCL20)in cells were increased(P＜0.05).The expres-sion level of phosphorylated nuclear factor-κB p65(p-p65)protein was increased(P＜0.01).After a certain con-centration of AE intervention,the viability of HaCaT cells was decreased(P＜0.01).Treatment with low,medium and high concentrations of AE in TNF-α pretreated HaCaT cells increased the early apoptosis rate and the mRNA-expression level of IκBα(P＜0.01).Meanwhile,it decreased the mRNA expression and release of IL-6,IL-1β,IL-17A,TNF-α and CCL20(P＜0.05),as well as the p-p65 protein level(P＜0.01).Conclusion:AE can inhibit the phosphorylation of p65 protein in the NF-κB pathway,thereby reducing the release of inflammatory factors and chemokines in the psoriatic-like inflammatory model of HaCaT cells,inducing apoptosis,and improving the in-flammatory response.关键词
沉香提取物/银屑病/HaCaT细胞/NF-κB信号通路/炎症因子/凋亡Key words
agarwood extract/psoriasis/HaCaT cells/NF-κB signaling pathway/inflammatory factors/apoptosis分类
中医学引用本文复制引用
黄薇薇,张琪,赵斌斌,李文宇,郭婷,温斯健,曹存巍,林有坤..沉香提取物通过调控NF-κB通路改善HaCaT细胞银屑病样炎症[J].广西医科大学学报,2025,42(2):247-254,8.基金项目
广西重点研发计划重点项目资助(No.桂科AB23026088) （No.桂科AB23026088）
