甘蔗糖业2025，Vol.54Issue(2)：69-75,7.DOI:10.3969/j.issn.1005-9695.2025.02.008
桂糖系列甘蔗品种试管苗生根过程的相关激素动态变化研究
Study on the Dynamic Changes of Relevant Hormones during the Rooting Process of in Vitro Seedlings of Guitang Series Sugarcane Varieties
摘要
Abstract
This study aims to precisely explore the dynamic change characteristics of relevant hormones in the rooting process of in vitro seedlings of the Guitang series sugarcane varieties,deeply analyze the internal action mechanism of hormones during the rooting process of sugarcane in vitro seedlings,and further provide a theoretical guidance for the optimization of the rooting culture scheme of sugarcane in vitro seedlings.Taking the tissue-cultured seedlings of the Guitang series as the research objects,the content changes of indole-3-acetic acid(IAA),zeatin riboside(ZR),and abscisic acid(ABA)were carefully determined during the three key stages,namely the rooting induction period(1～3 days),primordium formation period(4～7 days),and root system elongation period(8～28 days).The research results show that within the complete test cycle,the IAA content of the three varieties,Guitang42,Guitang47,and Guitang49,generally shows a gradually decreasing trend as a whole,while the ABA content significantly increases and reaches its peak value on the 28th day.At the same time,in the early stage of rooting,the ZR content of Guitang42,Guitang47,and Guitang49 remains in a state of relatively stable small fluctuations.However,with the continuous progress of the rooting process,the ZR content begins to rise continuously.In addition,this study also finds that during the entire test stage,the IAA/ABA ratio of the above three varieties generally shows a downward trend,and the IAA/ZR ratio also generally shows a downward trend within the entire test cycle.The findings of this study provide precise optimization for sugarcane tissue culture rooting conditions,such as supplementing exogenous hormones based on changes in endogenous hormone levels,and by understanding the hormonal changes during rooting and their relationship with root formation and survival,thereby providing technical support for the optimization of pre-transplantation hormone treatments.关键词
甘蔗/桂糖系列/组培苗/内源激素/吲哚乙酸/玉米素核苷/脱落酸Key words
Sugarcane/Guitang series/Tissue culture seedlings/Endogenous hormone/Indole-3-acetic acid/Zeatin riboside/Abscisic acid分类
农业科技引用本文复制引用
刘红坚,刘丽敏,何为中,刘俊仙,张荣华,黄杏,卢曼曼,陈荣发,范业赓..桂糖系列甘蔗品种试管苗生根过程的相关激素动态变化研究[J].甘蔗糖业,2025,54(2):69-75,7.基金项目
国家自然科学基金项目(32360485) （32360485）
广西自然科学基金(2023GXNSFAA026004) （2023GXNSFAA026004）
广西农业科学院科研项目(桂发科2021YT008) （桂发科2021YT008）
