海南大学学报（自然科学版）2025，Vol.43Issue(1)：25-32,8.DOI:10.15886/j.cnki.hdxbzkb.2024031701
丛生盔形珊瑚转录组微卫星标记的分离和鉴定
Development of novel EST-SSR markers for the stony coral Galaxea fascicularis
摘要
Abstract
Galaxea fascicularis is a representative species of reef-building coral in the South China Sea.Based on the nematocyst morphology and the indel genotype of CYTB-ND2 in mitochondrial genome,G.fascicularis can be divided into two morphotypes:S and H,and there is significant differentiation in the genetic and ecological phenotypes between these two morphotypes.By mining microsatellite resources from the transcriptome sequences,we developed 11 SSR markers that could be used in both morphotypes,and tested and validated them in one S population(n=16)and one H population(n=31),respectively.All the 11 markers showed polymorphism.In S population,the number of alleles ranged from 3 to 8(mean 4.8±1.9),the observed heterozygosity(HO)ranged from 0.06 to 0.92(0.55±0.26),and the expected heterozygosity(HE)ranged from 0.24 to 0.83(0.40±0.27).In H population,the number of alleles were 2～9(4.5±2.1),the observed heterozygosity(HO)was 0.17～1.00(0.70±0.15),and the expected heterozygosity(HE)was 0.46～0.86(0.62±0.19).Only one locus(Gf-E24)deviated from the Hardy-Weinberg equilibrium in H population,probably due to the presence of null allele.关键词
丛生盔形珊瑚/EST-SSR/形态型/线粒体基因型/遗传分化Key words
Galaxea fascicularis/EST-SSR/morphotypes/mitochondrial genotype/genetic differentiation分类
农业科技引用本文复制引用
卢章旺,李福宇,廖婷馨,李立微,王嫣..丛生盔形珊瑚转录组微卫星标记的分离和鉴定[J].海南大学学报（自然科学版）,2025,43(1):25-32,8.基金项目
国家科技基础资源调查专项课题(2022FY100604) （2022FY100604）
国家自然科学基金项目(41376174) （41376174）
