河北农业大学学报2025，Vol.48Issue(2)：26-36,11.DOI:10.13320/j.cnki.jauh.2025.0021
烟管菌生物学特性、驯化栽培及抗氧化活性
Biological characteristics,domestic cultivation and antioxidant activities of Bjerkandera adusta
摘要
Abstract
The strain of Bjerkandera adusta was isolated from wild fruiting body tissues collected in Zunhua City,Hebei Province.Biological characteristics were evaluated through single factor and orthogonal experiments.Domesticated fruiting bodies were cultivated from this strain.Scavenging ability of alcohol extracts from wild and cultivated fruiting bodies were determined against ABTs+,DPPH and·OH were carried out.The results showed that optimal carbon and nitrogen source,temperature,as well as pH for mycelial growth were fructose,yeast extract powder,30℃,and pH 6.Orthogonal experiments showed that temperature had the greatest effect on the growth of mycelium.The subordinate factors were carbon source,nitrogen source and pH.Domestication cultivation indicated that mycelia were sackful after 20 days;B.adusta needed 40～45 days to form primordium under the conditions of 300～500 lx of light,80%～85%humidity,25～30℃during the day and 16～18℃at night.Fruiting bodies matured in 15～20 days after humidity was increased to 90%～95%.Antioxidant activities of alcohol extracts of both wild and cultivated fruiting bodies were gradually increased as the additive amount raising.Scavenging rates of ABTs+,DPPH and·OH reached 84.10%,60.74%and 96.06%respectively in wild fruiting bodies when additive amount was 200 μL,which were significantily higher than cultivated fruiting bodies.Scavenging rates of cultivated fruiting bodies reached 70.54%,35.81%and 63.16%,respectively.The difference in the scavenging rate of above free radicals between wild and cultivated fruiting bodies were 13.56%,24.93%and 32.90%,respectively.This research provided a reference for further development and utilization of B.adusta.关键词
烟管菌/单因素/药用真菌/正交试验Key words
Bjerkandera adusta/single factor/medicinal fungi/orthogonal test分类
农业科技引用本文复制引用
康霞,常光雷,任静,王立安,李肖,李守勉,李国杰..烟管菌生物学特性、驯化栽培及抗氧化活性[J].河北农业大学学报,2025,48(2):26-36,11.基金项目
河北农业大学引进人才科研专项(YJ201849) （YJ201849）
国家食用菌产业技术体系小宗食用菌岗位(No.CARS-20) （No.CARS-20）
河北省科技支撑计划资助项目(2053731D) （2053731D）
河北省科技计划项目重点研发计划(21326315D) （21326315D）
河北省食用菌产业创新团队珍稀食用菌岗位(HBCT2023090202) （HBCT2023090202）
河北省县域生物多样性调查评估项目(2011600012). （2011600012）
