河北农业大学学报2025，Vol.48Issue(2)：46-53,8.DOI:10.13320/j.cnki.jauh.2025.0023
壳聚糖对甜瓜白粉病抗性的影响
Effect of chitosan on melon resistance to powdery mildew
摘要
Abstract
To explore the influence of chitosan on the resistance of melon to powdery mildew and its underlying mechanisms,the susceptible melon variety'105'was selected as the experimental material.When the seedlings reached the three leaves stage and one heart-shaped leaf stage,they were sprayed with chitosan at four concentrations:400,800,1 200,and 1 600 mg/L.Twenty-four hours after the chitosan treatment,the powdery mildew pathogen was inoculated onto the leaves using a spray method.The study investigated the effects of chitosan on the resistance of melon plants to powdery mildew and analyzed the changes in the antioxidant system,phenylpropanoid metabolism,and chlorophyll fluorescence parameters of the treated melon leaves.The results showed that compared with the control,the disease index of melon leaves treated with 800 mg/L chitosan was significantly reduced by 39.79％,with a relative control effect of 62.00％.The maximum photochemical efficiency and photosynthetic performance index were significantly increased.The activities of superoxide dismutase,peroxidase,catalase,polyphenol oxidase,and phenylalanine ammonia-lyase were significantly increased by 53.22％,17.97％,62.11％,64.15％,and 54.24％,respectively.Meanwhile,chitosan treatment could reduce the production rate of superoxide anion and the content of malondialdehyde,while increasing the contents of proline,soluble sugars,total phenols,and flavonoids.Correlation analysis indicated that the disease index was closely related with photosynthetic efficiency,antioxidant capacity,osmotic adjustment substances,and phenylpropanoid metabolism.Principal component analysis showed that muskmelon seedlings treated with 800 mg/L chitosan scored higher than the control,with the highest composite score,indicating the best disease resistance.In summary,foliar application of 800 mg/L chitosan can enhance the resistance of muskmelon leaves to powdery mildew by reducing the accumulation of reactive oxygen species,promoting phenylpropanoid metabolism,and enhancing photosynthetic capacity.关键词
甜瓜/白粉病/壳聚糖/防御酶/抗病性Key words
melon/powdery mildew/chitosan/defense enzymes/disease resistance分类
农业科技引用本文复制引用
曹艳霞,刘海河,张彦萍,朱立保,郑海强,刘梦泽,康凯伦,祁泽阳..壳聚糖对甜瓜白粉病抗性的影响[J].河北农业大学学报,2025,48(2):46-53,8.基金项目
国家西甜瓜产业技术体系资助项目(CARS-26-25) （CARS-26-25）
河北省现代种业科技创新专项资助项目(21326306D) （21326306D）
河北省农业科技成果转化资金专项资助项目(冀科农函[2024]1号). （冀科农函[2024]1号）
