河北农业大学学报2025，Vol.48Issue(2)：54-61,8.DOI:10.13320/j.cnki.jauh.2025.0024
小麦酪蛋白激酶TaCK2α启动子的克隆与启动活性分析
Cloning and activity analysis of the promoter of wheat casein kinase TaCK2α
摘要
Abstract
Casein kinase 2 is a ubiquitous constitutively active enzyme that is consistently involved in plant growth,development,and responses to environmental stresses.However,the function of CK2 playing in the interaction between wheat Puccinia triticina(Pt)is unclear.A casein kinase gene,TaCK2α,was identified based on transcriptome sequencing of TcLr19 wheat inoculated with Pt,indicating its involvement in wheat defense response against Pt.In order to further explore its regulatory mechanism,the promoter sequence of TaCK2α was cloned according to the sequence of China Spring wheat database which was named as pTaCK2α.The cis-regulatory elements of TaCK2α promoter were predicted by PlantCARE,and the results showed that TaCK2α promoter contained core elements,stress response elements,hormone response elements and light response elements.The fusion vectors pTaCK2α-pBI121,pTaCK2α-pCamA carrying β-Glucuronidase and Green fluorescent protein reporter gene driven by pTaCK2α were constructed and transferred into tobacco via Agrobacterium-mediated method.GUS histochemical staining and GFP fluorescence observation showed that the promoter of TaCK2α gene has promoter activity.Taken together,these results lay a solid foundation for exploring the regulation mechanism of TaCK2α involved in wheat defense response against Pt.关键词
小麦叶锈菌/酪蛋白激酶2α/顺式作用元件/启动子/启动活性Key words
Puccinia triticina/casein kinase 2α/cis-acting element/promoter/promoter activity分类
农业科技引用本文复制引用
冯燕,吴志会,曹雨欣,申松松,崔钟池,丁大烁,王海燕,刘大群..小麦酪蛋白激酶TaCK2α启动子的克隆与启动活性分析[J].河北农业大学学报,2025,48(2):54-61,8.基金项目
国家自然科学基金(32172384 （）
31501623) （）
河北省自然科学基金(C2020204028) （C2020204028）
中央引导地方科技发展资金项目(246Z6504G). （246Z6504G）
