河北农业大学学报2025，Vol.48Issue(2)：70-79,10.DOI:10.13320/j.cnki.jauh.2025.0026
负载牛干扰素α/γ聚合物纳米颗粒的制备及其抗病毒活性评价
Preparation of polymer nanoparticles loaded with bovine interferon α/γ and evaluation of their antiviral activity
摘要
Abstract
Calf diarrhea caused by bovine viral diarrhea virus(BVDV)infection causes great economic losses to the cattle industry.In order to develop effective preventive and curative drugs,this study utilized a prokaryotic expression system for the fusion expression of bovine interferon α(BoIFNα)and bovine interferon γ(BoIFNγ),and recombinant bovine interferon fusion protein(rBoIFNα/γ)was successfully expressed and purified.In νitro experiments showed that rBoIFNα/γ had low toxicity and high antiviral effects,with anti-BVDV activity up to 9 292 U/mL.To further improve the antiviral effect and drug duration of action,polymer nanoparticles(PEG-PCL-rBoIFNα/γ NPs)were formed by encapsulating them with poly(ethylene glycol)-poly(caprolactone)(PEG-PCL)and evaluated for in νiνo and in νitro activities.The results showed that the PEG-PCL-rBoIFNα/γ NPs were spherical particles with a diameter of around 169.09 nm,which had no significant toxic effects on cells and were released continuously for 96 h in biological fluids.In νiνo experiments showed that injection of PEG-PCL-rBoIFNα/γ NPs could effectively alleviate the symptoms and tissue damage in mice,reduce the viremia level and tissue viral load,and inhibit the expression of inflammatory factors.The findings provide new ideas for the prevention and treatment of BVDV.关键词
牛干扰素/融合蛋白/聚合物纳米颗粒/抗病毒/牛病毒性腹泻病毒Key words
bovine interferon/fusion protein/polymeric nanoparticles/antiviral/bovine viral diarrhea virus分类
农业科技引用本文复制引用
冯晶晶,刘国锰,雷白时,袁万哲,张武超,冯小宇,赵款..负载牛干扰素α/γ聚合物纳米颗粒的制备及其抗病毒活性评价[J].河北农业大学学报,2025,48(2):70-79,10.基金项目
河北省自然科学基金生物农业联合基金重点项目(C2022204249) （C2022204249）
农业农村部兽用生物制品工程技术重点实验室开放课题(SYKF(23)001) （SYKF(23）
国家兽用生物制品工程技术研究中心开放课题(GTKF(24)006). （GTKF(24）
