湖北农业科学2025，Vol.64Issue(4)：24-30,7.DOI:10.14088/j.cnki.issn0439-8114.2025.04.005
基于ImageJ的药用植物黄精染色体核型分析
Karyotype analysis of medicinal plant Polygonatum sibiricum based on ImageJ
摘要
Abstract
Using the medicinal plant Polygonatum sibiricum as an example,a karyotype analysis method and workflow for plant chro-mosomes was established based on the open-source image processing software ImageJ.The ImageJ software was opened,and the Stow-ers plugin package and LEVAN plugin were loaded.The acquired microscopic images of Polygonatum sibiricum metaphase chromo-somes were processed through image preprocessing,chromosome contour selection,chromosome segmentation and debris removal,and chromosome cropping to obtain chromosome morphological data.Photoshop software was used for karyotype arrangement,and Ex-cel software was used to draw the karyotype ideogram.The results showed that,according to Levan's karyotype classification stan-dard,the karyotype formula of Polygonatum sibiricum was 2n=24=4m+10sm+10st,with an asymmetry index of 72.11%,belong-ing to the 3B type.This method proposed a solution strategy for chromosome contact or overlap in karyotype images,with the advantag-es of being open-source,free,having a clear operational workflow,and providing accurate interaction.关键词
黄精(Polygonatum sibiricum)/ImageJ/药用植物/染色体/核型分析Key words
Polygonatum sibiricum/ImageJ/medicinal plants/chromosomes/karyotype analysis分类
农业科技引用本文复制引用
游磊,朱哲慧,石宪铭,张泽志,郑兰兰,张勇洪..基于ImageJ的药用植物黄精染色体核型分析[J].湖北农业科学,2025,64(4):24-30,7.基金项目
国家自然科学基金项目(32000609 （）
32200680) （）
湖北医药学院PI项目(HBMUPI202104) （HBMUPI202104）
湖北省科技计划项目(2022BCE035) （2022BCE035）
十堰市科技研发重点项目(2021K20) （2021K20）
