湖北农业科学2025，Vol.64Issue(4)：51-57,121,8.DOI:10.14088/j.cnki.issn0439-8114.2025.04.010
黄淮麦区小麦品种(系)品质与抗性基因的KASP分型检测
Detection of quality and resistance genes of wheat varieties(lines)in Huang-Huai wheat region by KASP genotyping technology
摘要
Abstract
To elucidate the genetic basis of quality and resistance traits in the dominant wheat(Triticum aestivum L.)varieties from the Huang-Huai wheat region,99 wheat varieties(lines)were genotyped using 12 KASP markers related to quality,disease resis-tance,drought resistance and pre-harvest sprouting resistance.The results revealed that 33 materials carried the Glu-D1d gene encod-ing high-quality gluten subunit 5+10,and 39 materials contained the Glu-A1 gene encoding Ax1/Ax2* strong-gluten haplotype.In terms of disease resistance,only 12 materials exhibited Lr68,a leaf rust resistance gene,and none of the tested materials were found to carry the Fhb1 gene for Fusarium head blight resistantce.For sprout resistance,the haplotypes TaSdr-B1a,Rio Blanco type,and Hap-H associated with pre-harvest sprouting were detected in 9,73,and 13 materials,respectively.In drought resistance,there were 53 materials carried 3Ba drought resistant haplotype of the COMT-3B gene,45 materials possessed the TaDREB-B1a drought resistance haplotype,and 89 materials carried the A2a drought resistance haplotype of the TaSST-4A gene.It could be seen that the drought resistance genes detected in wheat varieties in Huang-Huai wheat region were widely distributed and accounted for a relatively high proportion,while the excellent haplotypes in disease resistance accounted for a relatively small proportion.Therefore,it was ur-gent to strengthen the introduction and utilization of disease resistance genes in breeding strategies.关键词
小麦(Triticum aestivum L.)/品质/抗病/抗旱/抗穗发芽/基因检测/KASP分型技术/黄淮麦区Key words
wheat(Triticum aestivum L.)/quality/disease resistance/drought resistance/pre-harvest sprouting resistance/gene de-tection/KASP genotyping technology/Huang-Huai wheat region分类
农业科技引用本文复制引用
王志,李寒冰,王伟,张玉杰,田蜜,王伟伟..黄淮麦区小麦品种(系)品质与抗性基因的KASP分型检测[J].湖北农业科学,2025,64(4):51-57,121,8.基金项目
河北省自然科学基金专项(C2023205050) （C2023205050）
河北省耐盐碱作物种业科技创新团队项目(23327501D) （23327501D）
国家现代农业产业技术体系项目(CARS-03) （CARS-03）
