江汉大学学报（自然科学版）2025，Vol.53Issue(2)：26-33,8.DOI:10.16389/j.cnki.cn42-1737/n.2025.02.003
藜蒿ISSR分子标记遗传多样性评价
Genetic Diversity Evaluation of ISSR Molecular Markers in Artemisia selengensis
摘要
Abstract
Artemisia selengensis is a perennial herb with a wide distribution,complex genetic background,and ambiguous systematic classification.The phylogenetic relationships of different populations of Artemisia selengensis should be understood to lay the foundation for genetic improvement.This study used ISSR molecular marker technology to comprehensively analyze 117 germplasm resources of Artemisia selengensis collected from different regions in 9 provinces of China.Using 9 ISSR primers,87 polymorphic loci were detected,with a polymorphism percentage of 98.86%.The average Nei's genetic diversity index(H)between different populations was 0.099 6,and the average Shannon genetic diversity index was 0.152 2.UPGMA cluster analysis grouped the 117 Artemisia selengensis samples into three main clusters at a genetic coefficient of 0.35.Among them,most samples belonged to the second cluster,five samples numbered 41-44 and 110 were classified into the first cluster,and the sample numbered 47 formed the third cluster alone.The different germplasm resources of Artemisia selengensis have close phylogenetic relationships and high genetic similarity indices.关键词
藜蒿/ISSR/分子标记/遗传多样性/聚类分析Key words
Artemisia selengensis/ISSR/molecular markers/genetic diversity/cluster analysis分类
生物学引用本文复制引用
陈美珠,董笑笑,夏忙,王宇航,曾长立..藜蒿ISSR分子标记遗传多样性评价[J].江汉大学学报（自然科学版）,2025,53(2):26-33,8.基金项目
湖北省重点研发计划项目(2022BBA0064) （2022BBA0064）
武汉市产学研项目(CXY202203) （CXY202203）
江汉大学一流学科建设重大科研专项(2023XKZ026) （2023XKZ026）
