江汉大学学报（自然科学版）2025，Vol.53Issue(2)：34-42,9.DOI:10.16389/j.cnki.cn42-1737/n.2025.02.004
玉米杂交种瑞华玉288指纹图谱构建及其纯度鉴定
Construction of Maize Hybrid Ruihuayu 288 Fingerprint and Its Purity Identification
摘要
Abstract
To construct the fingerprint of the new hybrid corn variety Ruihuayu 288,the InDel primers suitable for its purity identification were screened.The fingerprint was constructed with InDel molecular marker technology,the purity of Ruihuayu 288 seeds was identified with the primers,and the identification results were verified by combining the field community planting identification method.The results showed that 20 pairs of primers with good polymorphism,clear,and stable primers were screened out of 52 pairs of InDel primers to construct the fingerprint of Ruihuayu 288,and the polymorphism was 38.46%.Eleven pairs of primers in 20 pairs of primers could be used for the purity identification of maize hybrids Ruihuayu 288,and the purity of Ruihuayu 288 identified with 3 pairs of primers was 96.26%,which was highly consistent with the results of 98.13%of the field community planting identification.The results indicate that the molecular identification method can quickly identify the purity of maize hybrids,which is conducive to the monitoring of maize seed quality.关键词
分子标记/遗传指纹图谱/玉米纯度/瑞华玉288Key words
molecular markers/genetic fingerprint/corn purity/Ruihuayu 288分类
农业科技引用本文复制引用
杜雨晴,王歆,杨子腾,刘海浪,任仰涛..玉米杂交种瑞华玉288指纹图谱构建及其纯度鉴定[J].江汉大学学报（自然科学版）,2025,53(2):34-42,9.基金项目
安徽科技学院江苏瑞华农业科技有限公司研究生企业工作站项目(2023qygzz032) （2023qygzz032）
