计量学报2025，Vol.46Issue(3)：433-441,9.DOI:10.3969/j.issn.1000-1158.2025.03.18
酿酒酵母标准物质的研制与应用
Research and Application of Saccharomyces Cerevisiae Reference Materials
摘要
Abstract
In order to solve the problem that the concentration of saccharomyces cerevisiae reference material prepared in the existing research reports can not meet the demand of aerosol generation during the sampling of plankton sampler,we developed a cerevisiae reference material for the measurement and evaluation of the sampling bioefficiency of plankton sampler.The results showed that the survival rate of yeast in the prepared saccharomyces cerevisiae reference materials reached 81.2±1.4%.The quantitative standard value of this reference material was 8.65×10⁶ CFU/vial,and the relative expanded uncertainty(Urel)was 4.32%(k=2).It had good homogeneity and stability and could be stably stored for 14 days at 4℃ and-20℃,and for 6 months at-80 ℃.In addition,the bioaerosol generated by the saccharomyces cerevisiae reference materials could be collected and maintain its activity,which could meet the requirements for the evaluation of the sampling biological efficiency of the airborne microorganism sampler.关键词
采样生物效率/计量评价/酿酒酵母/浮游菌采样器/标准物质Key words
biological sampling efficiency/metrological evaluation/saccharomyces cerevisiae/planktonic bacteria sampler/reference material分类
通用工业技术引用本文复制引用
刘乃毓,李曼莉,傅博强,李茹..酿酒酵母标准物质的研制与应用[J].计量学报,2025,46(3):433-441,9.基金项目
中国计量科学研究院产品服务项目(CPF4622050) （CPF4622050）
