计量学报2025，Vol.46Issue(3)：450-458,9.DOI:10.3969/j.issn.1000-1158.2025.03.20
微生物组绝对定量标准物质研究进展
Research Advances of Reference Materials in Absolute Quantification of Microbiome
摘要
Abstract
Microbiome research based on amplicon and metagenomic sequencing can help to analysis microbial community composition and function,and is an important tool for the study of microbial structure ecology and interaction mechanism.In order to represent the host-microbiome interactions,microbiome sequencing is gradually changing from relative abundance to absolute quantitative analysis of community composition.Reference material can be used as a standardized factor to accurately quantify the total load of each microorganism in the community.The reference materials for absolute quantification of microbiome,including microbial consortia,microbial genome,internal standard strains and spike-in sequences,and their applications are reviewed in this paper,the development trend of reference materials in quality control and standardization of microbiome is also proposed,so as to call for further expanding the application range of reference materials for absolute quantification for microbiome in microbial ecology.关键词
生物计量/微生物组/肠道微生物/标准物质/高通量测序/绝对定量Key words
biometrology/microbiome/gut microbiome/reference material/NGS/absolute quantification引用本文复制引用
张瑜,王霞,牛春艳,张永卓,杨佳怡,董莲华..微生物组绝对定量标准物质研究进展[J].计量学报,2025,46(3):450-458,9.基金项目
中国计量科学研究院基本科研业务费探索性创新项目(AKYCX2407) （AKYCX2407）
