江苏农业学报2025，Vol.41Issue(3)：445-456,12.DOI:10.3969/j.issn.1000-4440.2025.03.003
玉米氮吸收性状相关NPF家族基因挖掘及InDel分子标记开发
Mining of NPF family genes related to maize nitrogen absorption traits and development of InDel molecular markers
摘要
Abstract
As the largest grain crop in China,maize plays an important role in ensuring food security in our country.Nitrogen fertilizer is an important fertility factor in the growth and development of maize.However,in recent years,a series of environmental problems have been caused by a large amount of nitrogen fertilizer application.Molecular marker-assisted breeding which gives full play to nitrogen use efficiency is an environmentally friendly and efficient method for solving these problems.In this study,the NPF family related to nitrate nitrogen absorption and transport was analyzed,and 15 candidate genes closely associated with maize nitrogen absorption traits were dug out through genome-wide association analysis.Insertion-deletion(InDel)molecular markers were designed according to the reported functional sites,to design functional markers significantly associated with maize nitrate accumulation traits.A total of 69 pairs of In-Del molecular markers were designed,and 32 pairs of markers with good specificity were tested by parental poly-morphism.The functional validation of these InDel molecu-lar markers in the natural population composed of 609 maize inbred lines showed that seven pairs of InDel molecular markers near the maize ZmNPF6.2,ZmNPF6.4,ZmNPF6.8,ZmNPF8.12 and ZmNPF8.14 genes were closely associated with nitrogen absorption traits.The InDel molecular markers re-lated to efficient nitrogen absorption developed in this study can provide a theoretical basis for molecular marker-assisted breeding and guide the direction for breeding new maize varieties with efficient nitrogen utilization traits.关键词
玉米/氮高效吸收/功能标记/InDel分子标记Key words
maize/efficient nitrogen absorption/functional marker/InDel molecular marker分类
农业科技引用本文复制引用
罗春梅,赵晨旭,余海兵,赵涵,周玲..玉米氮吸收性状相关NPF家族基因挖掘及InDel分子标记开发[J].江苏农业学报,2025,41(3):445-456,12.基金项目
国家自然科学基金项目(32272133、32372031、32101644) （32272133、32372031、32101644）
江苏省种业振兴"揭榜挂帅"项目[JBGS(2021)012] （2021）
江苏省重点研发计划(现代农业)项目(BE2022343) （现代农业）
