江苏农业学报2025，Vol.41Issue(3)：457-467,11.DOI:10.3969/j.issn.1000-4440.2025.03.004
基于转录组测序筛选烟草响应疫霉侵染的候选基因
Screening of candidate genes in response of tobacco to Phytophthora nicoti-anae infection based on transcriptome sequencing
摘要
Abstract
Phytophthora fungi are important plant pathogens that cause devastating damage to many crops.To identify candidate genes in tobacco that respond to Phytophthora nicotianae infection,seedlings of Heluo No.1 were infected with Phytoph-thora nicotianae.Differential expression genes(DEGs)before infection and 24 h,48 h after infection were analyzed by Gene on-tology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses,based on transcriptome sequencing.The results were verified by quantitative real-time polymerase chain reaction(qRT-PCR).The results indicated that 4563 DEGs and 14 053 DEGs were identified at 24 h and 48 h post-infection,respectively,with 3 623 genes showing common changes.A-mong them,1 502 up-regulated DEGs were annotated by GO genome,mainly enriched in glutathione metabolism process and pro-tein phosphorylation biological process.A total of 787 down-regulated DEGs were annotated by GO genome,mainly enriched in transcriptional regulation.KEGG enrichment analysis showed that DEGs were primarily enriched in the plant-mitogen activated protein kinase signaling pathway,plant hormone signal transduction pathway and plant-pathogen interaction pathway.In the plant-pathogen interaction pathway,the up-regulation of FLS2 promoted the phosphorylation of MEKK1,which induced the expression of protein kinase MPK4,caused the continuous down-regulation of WRKY12 and the continuous up-regulation of WRKY9,WRKY24,WRKY26,WRKY31,WRKY33 and WRKY40.Besides,the expression of protein kinases MPK3/6 was induced,leading to the up-regulation of WRKY11,WRKY15,WRKY17 and WRKY41,and the continuous down-regulation of the PR-1 gene.As a result,Heluo No.1 plants were more susceptible to Phytophthora nicotianae infection.关键词
转录组/疫霉/河洛1号/烟草Key words
transcriptome/Phytophthora nicotianae/Heluo No.1/tobacco分类
农业科技引用本文复制引用
孙计平,王亚乐,李雪君,平文丽,孙焕..基于转录组测序筛选烟草响应疫霉侵染的候选基因[J].江苏农业学报,2025,41(3):457-467,11.基金项目
河南省烟草公司洛阳市公司科技项目(202241030020005-3) （202241030020005-3）
河南省农业科学院基础性科研工作项目(2024JC09) （2024JC09）
