江苏农业学报2025,Vol.41Issue(3):510-516,7.DOI:10.3969/j.issn.1000-4440.2025.03.010
绿豆腐霉根腐病抗性鉴定技术体系构建及抗病资源筛选
Establishment of resistance identification technology system for mung bean Pythium myriotylum root rot and screening of resistant resources
摘要
Abstract
The mung bean root rot caused by Pythium myriotylum is a recently reported soil-borne disease that presents a considerable pathogenic hazard to mung bean.To establish a laboratory technique for assessing resistance of mung bean a-gainst P.myriotylum and achieve rapid and effective screening of disease-resistant resources,three inoculation methods inclu-ding root irrigation inoculation method,mycelial layer inoculation method and hypocotyl wound inoculation method were used to evaluate and compare the resistance of mung bean to P.myriotylum.The results showed that the mycelial layer inoculation method had the advantages of simple operation,short test period,and stable results.To screen mung bean germplasm re-sources with resistance to root rot disease caused by P.myriotylum,394 collected mung bean germplasm resources were as-sessed using the mycelial layer inoculation method.The resultsshowed that 98.48%(388 samples)of germplasms exhibited medium to high susceptibility,with disease index(DI)>40.0,only six germplasms(1.52%)exhibited in-termediate resistance(20.0<DI≤40.0),and none showed resistance or immunity(DI≤20.0).The results indicated that the collected mung bean germplasms with resistance to P.myriotylum were scarce.In this study,we established a potential method for identifying resistance resources of mung bean against root rot disease caused by Pythium species.关键词
绿豆/群结腐霉/根腐病/抗性评价/抗病资源Key words
mung bean/Pythium myriotylum/root rot/resistance evaluation/resistant resources分类
农业科技引用本文复制引用
闫强,刘欣悦,袁星星,陈新,刘欢,王瑞敏,薛冬,周琰琰,郑豫,武婷,任亚举,陈雨婷..绿豆腐霉根腐病抗性鉴定技术体系构建及抗病资源筛选[J].江苏农业学报,2025,41(3):510-516,7.基金项目
国家食用豆产业技术体系生物防治与综合防控岗位科学家项目(CARS-08-G15) (CARS-08-G15)
江苏省种业揭榜挂帅项目[JBGS(2021)004] (2021)
种质资源精准鉴定评价项目(005012691230229) (005012691230229)