菌物学报2025，Vol.44Issue(4)：29-43,15.DOI:10.13346/j.mycosystema.240279
禾谷镰孢菌bZIP转录因子FgMetR的生物学功能
Biological functions of the bZIP transcription factor FgMetR in Fusarium graminearum
摘要
Abstract
Fusarium head blight(FHB)caused by Fusarium graminearum is one of the most important fungal diseases of wheat,posing a serious threat to wheat production and food safety.bZIP transcription factors are core positive regulators of sulfur assimilation and involved in the regulation of fungal asexual reproduction and pathogenicity.In order to further reveal the biological function of the bZIP transcription factors in F.graminearum,in this study,the authors identified a bZIP transcription factor FgMetR in F.graminearum and obtained the deletion mutant△FgMetR.The effect of FgMetR deletion on various phenotype was evaluated.The results showed that the colony of △FgMetR appeared transparent on PDA plates and the mycelia were sparsely dispersed with few aerial hyphae.No red pigment was observed either on PDA plates or in PDB broth.The radial growth rate of colonies,mycelial height,and conidial production of△FgMetR were significantly reduced as compared with those of the wild-type strain.The growth defect phenotype of △FgMetR on minimal medium without any sulfur element can be partially restored by supplementing organic but not inorganic sulfur sources.△FgMetR mutant failed to form perithecia and ascospores on carrot culture medium.Deletion of FgMetR significantly reduced the pathogenicity of F.graminearum invading both wheat coleoptiles and heads.In addition,△FgMetR mutant was more sensitive to tebuconazole and prochloraz.The facts prove that bZIP transcription factor FgMetR is essential for the vegetative growth,pigmentation,asexual and sexual reproduction,pathogenicity and involvement in the regulation of sulfur assimilation and sensitivity to azole fungicides of F.graminearum.关键词
赤霉病/转录调控/硫同化/致病力/唑类杀菌剂Key words
Fusarium head blight/transcriptional regulation/sulfur assimilation/pathogenicity/azole fungicide引用本文复制引用
马浩,张丽敏,赵彦翔,黄金光..禾谷镰孢菌bZIP转录因子FgMetR的生物学功能[J].菌物学报,2025,44(4):29-43,15.基金项目
国家重点研发计划(2023YFD2303200,2022YFD1400104) （2023YFD2303200,2022YFD1400104）
山东省自然科学基金(ZR2022QC152)This work was supported by the National Key Research and Development Program of China(2023YFD2303200,2022YFD1400104)and the Shandong Provincial Natural Science Foundation(ZR2022QC152). （ZR2022QC152）
