菌物学报2025，Vol.44Issue(4)：44-58,15.DOI:10.13346/j.mycosystema.240214
基于基因组重测序开发中华包革耳(金耳菌)菌株的鉴别标记
Identification markers for Naematelia sinensis strains based on genome resequencing
摘要
Abstract
Using the genome of Naematelia sinensis monospore strain NX-20 as the reference,resequencing data from four monospore strains(D13,D25,D40,and D47)with different mating types was analyzed by using bioinformatics software to identify SNP and InDel positions.Primer5.0 software was used to design SNP/InDel marker primers,resulting in 8 pairs of SNP and 6 pairs of InDel markers with polymorphic differences for genetic diversity analysis of 14 widely used N.sinensis strains in the cultivation industry.Results of SNP marker analysis revealed that the J2F1 strain exhibited two genotypes in eight pairs of SNP primers,while other strains showed only one genotype.The analysis results of 6 pair InDel markers showed that the J2F1 strain displayed unique band patterns in the 1st,2nd,and 5th pairs of primers compared to other strains,while most strains(such as YL,YR,JB,LL,and SC)exhibited consistent band patterns,indicating the phenomenon of synonyms.These findings enhance our understanding of the genetic diversity of N.sinensis cultivated germplasm resources,and the identified SNP and InDel molecular markers can serve as valuable tools for genetic analysis,germplasm resource identification,protection and molecular-assisted breeding in N.sinensis research.关键词
中华包革耳/单孢/重测序/插入缺失标记/单核苷酸多型性标记Key words
Naematelia sinensis/monospore/re-sequencing/InDel marker/SNP marker引用本文复制引用
沈真辉,杨林雷,曹瑶,罗祥英,杨爱霞,陆青青,子灵山,李荣春..基于基因组重测序开发中华包革耳(金耳菌)菌株的鉴别标记[J].菌物学报,2025,44(4):44-58,15.基金项目
云南省张劲松专家工作站(202305AF150084) （202305AF150084）
云南昆明张劲松药用真菌专家工作站(YSZJGZZ-2022043)This work was supported by the Yunnan Province ZHANG Jingsong Expert Workstation(202305AF150084)and the Yunnan(Kunming)ZHANG Jingsong Medicinal Fungus Expert Workstation(YSZJGZZ-2022043). （YSZJGZZ-2022043）
