菌物学报2025，Vol.44Issue(4)：68-80,13.DOI:10.13346/j.mycosystema.240271
铜离子对黑木耳漆酶活性及其基因转录水平的影响
Effects of copper ions on the laccase activities and gene transcription level of Auricularia heimuer
摘要
Abstract
The effect of copper ions on laccase activity and transcriptional expression of laccase genes in Auricularia heimuer"Hei Hou Yuan"is investigated.The results show that as the copper ion concentration increases from 100 to 700 μmol/L,extracellular laccase activity in A.heimuer increases significantly.Copper ion concentration strongly influences the transcriptional levels of laccase genes.Specifically,the transcription levels of AhLac1,AhLac3,and AhLac6 are significantly upregulated as the copper ion concentration rises.The expression levels of AhLac2,AhLac4,and AhLac5 peak at 500 μmol/L,and increase by 3.38-,1.29-,and 1.59-fold,respectively.Under different induction times,the transcription levels of AhLac1,AhLac3,and AhLac6 show varied degrees of upregulation with the extension of induction period.Correlation analysis further reveals a positive relationship between laccase activity and the transcriptional levels of AhLac1,AhLac3,and AhLac6.This study provides a scientific basis for improving the yield of A.heimuer and investigating the molecular regulatory mechanisms underlying laccase gene expression.关键词
黑木耳/铜离子/漆酶活性/转录表达Key words
Auricularia heimuer/copper ion/laccase activity/transcription level引用本文复制引用
米昊雨,耿楠楠,杜鹏禹,刘增才,邹莉..铜离子对黑木耳漆酶活性及其基因转录水平的影响[J].菌物学报,2025,44(4):68-80,13.基金项目
山东省重点研发计划(2024LZGCQY018)This work was supported by the Key Research and Development Program of Shandong Province(2024LZGCQY018). （2024LZGCQY018）
