菌物学报2025，Vol.44Issue(4)：81-97,17.DOI:10.13346/j.mycosystema.240258
金针菇Fvpal1基因的RNAi降低PAL酶活及菌丝色素分泌
RNAi of the Flammulina filiformis Fvpal1 gene reduces PAL enzyme activities and mycelial pigment secretion
摘要
Abstract
RNA interference for the phenylalanine deaminase gene 1 of Flammulina filiformis(F.filiformis PAL gene1,Fvpal1)was constructed,and five gene-silenced mononuclear transformants(RNAi-Fvpal1 1-5)were obtained through genetic transformation by using the mononuclear strain 0990-⑤(yellow)as the receptor.The five transformants were separately crossed with monokaryotic strain Dan3(white)to obtain five gene-silenced binuclear transformants(ZRNAi-Fvpal1 1-5).The Fvpal1 gene expression and traits of the transformants such as mycelial growth rate in PDA medium,PAL enzyme activity,and mycelial pigment secretion were further investigated to verify that Fvpal1 gene has the function of regulating the color of F.filiformis.The results showed that Fvpal1 gene expression was significantly down-regulated in all 10 transformants as compared with that in wild strain(P＜0.05).Mononuclear transformants RNAi-Fvpal1 1-5 were down-regulated respectively by 83.41％,75.92％,79.69％,66.49％,and 43.22％,and binuclear transformants ZRNAi-Fvpal1 1-5 were down-regulated respectively by 80.26％,45.24％,34.09％,84.05％,and 79.62％.All nine transformants except the binuclear transformant ZRNAi-Fvpal1 4 had significantly lower PAL-ase activity than the original strain 0990-⑤(P＜0.05).The mycelial pigment secretion of all the 10 transformants grown on PDA medium was lighter than that of the original strain,and the colour of the binucleate transformants became lighter even more significantly on woodchip medium.The fruiting body color of the binuclear transformants is also lighter than that of the strain 0990-⑤.In this study,the RNAi system of Fvpal1 gene of F.filiformis was constructed,and it was found that this gene had a positive regulatory effect on the color of mycelium and fruiting body of F.filiformis.The results provide preliminary evidence in support of further functional gene research of Fvpal1 of F.filiformis.关键词
金针菇/Fvpal1基因/RNA干扰/颜色Key words
Flammulina filiformis/Fvpal1 gene/RNA interference/colour引用本文复制引用
陆欢,沈玲,刘建雨,尚晓冬,王瑞娟,谭琦,唐桂容..金针菇Fvpal1基因的RNAi降低PAL酶活及菌丝色素分泌[J].菌物学报,2025,44(4):81-97,17.基金项目
上海市农业科学院卓越团队建设计划(沪农科卓[2022]001) （沪农科卓[2022]001）
上海市农业科学院助跑计划(ZP24161)This work was supported by the SAAS Program for Excellent Research Team([2022]001)and the SAAS Program for Acceleration Plan(ZP24161). （ZP24161）
