菌物学报2025，Vol.44Issue(4)：98-107,10.DOI:10.13346/j.mycosystema.240268
粗糙脉孢菌中组氨酸激酶响应香芹酚胁迫的作用机制
Mechanism of histidine kinases in responses to carvacrol stress in Neurospora crassa
摘要
Abstract
The role of histidine kinases(HKs)in response to carvacrol stimulation in Neurospora crassa was investigated by comparing the morphological changes,the formation of growth-inhibition zone,total superoxide dismutase(T-SOD)and catalase(CAT)activities,as well as carotenoid content between different hk-deficient mutants and the wild-type N.crassa under carvacrol stress.hk-deficient strains,with the exception of △dcc1,exhibited a zone of growth-inhibition and increased malondialdehyde(MDA)synthesis under carvacrol stress.Analysis of T-SOD and CAT activities revealed that the deletion of hcp1 and sln1 resulted in inhibition of T-SOD activity,while the deletion of os1,phy2,and hk1 led to reduction of CAT activity.Carvacrol stress stimulated T-SOD activity in the wild-type strain and △hcp1,△sln1,△dcc1,and △hk16 mutants.However,it did not induce CAT activity in the strains △hcp1,△nik2,△phy2,△luxq,△dcc1,and △hk16.Carotenoids are the main components within N.crassa and have antioxidant properties.It was observed that the deletion of hk9,os1,sln1,phy1,phy2,luxq,hk1,and dcc1 resulted in inhibition of carotenoid synthesis,but the carvacrol stress promoted the synthesis of carotenoids in △sln1,△luxq,and △hk16 mutants.In summary,N.crassa responded to carvacrol stress by regulating the activities of T-SOD and CAT and the synthesis of carotenoids through HKs which sense the signal from carvacrol.关键词
组氨酸激酶/香芹酚/类胡萝卜素/丙二醛/超氧化物歧化酶Key words
histidine kinase/carvacrol/carotenoids/malondialdehyde/superoxide dismutase引用本文复制引用
陈鹏旭,蓝子依,席娟,陈莹莹,郑维发,赵艳霞..粗糙脉孢菌中组氨酸激酶响应香芹酚胁迫的作用机制[J].菌物学报,2025,44(4):98-107,10.基金项目
国家自然科学基金(31971564) （31971564）
江苏师范大学2024年研究生科研与实践创新计划项目(2024XKT1512)This work was supported by the National Natural Science Foundation of China(31971564)and the 2024 Graduate Research and Practice Innovation Program Project of Jiangsu Normal University(2024XKT1512). （2024XKT1512）
