菌物学报2025，Vol.44Issue(4)：108-122,15.DOI:10.13346/j.mycosystema.240254
不同栽培基质对银耳子实体分级醇沉粗多糖结构特征及活性的影响
Effects of different cultivation substrates on the structural characteristics and activities of graded alcohol precipitated crude polysaccharides of Tremella fuciformis
摘要
Abstract
Six crude polysaccharide fractions,BC-50,BC-70,MZK-50,MZK-70,DM-50,and DM-70,were obtained by graded alcohol precipitation with 50％and 70％ethanol from Tremella fuciformis cultivated on Bentham's cress(BC)substrate and cottonseed husk(MZK)substrate in Gutian,Fujian Province,and on cut-log(DM)substrate in Tongjiang,Sichuan Province.The polysaccharide content,protein content,glucuronic acid content,molecular weight distribution,monosaccharide composition,antioxidant activity and immunological activity of the crude polysaccharide fractions from different matrix sources were analyzed and studied.The results showed that the highest polysaccharide content appeared in DM-70 fraction,reaching(80.68±0.23)％;the highest crude polysaccharide yield obtained in BC-70 fraction,reaching 14.27％;the highest glyoxylate content appeared in BC-50 fraction,reaching(21.04±0.35)％;the molecular weight distribution of 70％alcohol-precipitated polysaccharides of T.fuciformis on Bentham's cress substrate was comparatively small,being 8.098×104 Da,and that of 50％alcohol-precipitated polysaccharides of T.fuciformis on cut-log was 2.184×106 Da.The 50％alcohol-precipitated polysaccharide was mainly composed of fucose,glucose,xylose,mannose and glucuronic acid,while the 70％alcohol-precipitated polysaccharide was mainly composed of fucose,xylose,mannose and glucuronic acid.In vitro antioxidant and immune activity tests showed that the 50％alcohol-precipitated polysaccharide fraction of T.fuciformis cultivated on Bentham's cress substrate had a higher free radical scavenging rate of DPPH and ABTS at an action concentration of 2 mg/mL.It was also found that the polysaccharide fraction had the strongest ability to stimulate the release of NO from macrophage cells in vitro.The in vitro repair activity test on UVB-damaged HSF cells showed that the BC-70 fraction had the best effect on HSF cells from UVB damage.关键词
栽培基质/分级醇沉/结构特性/抗氧化活性/体外免疫活性Key words
cultivated substrate/graded alcohol deposition/structural characterization/antioxidant activity/in vitro immunoreactivity引用本文复制引用
叶宇航,陈万超,吴迪,刘朋,张忠,李文,王元凤,杨焱..不同栽培基质对银耳子实体分级醇沉粗多糖结构特征及活性的影响[J].菌物学报,2025,44(4):108-122,15.基金项目
上海现代农业产业技术体系[沪农科产字(2024)第9号]This work was supported by the Shanghai Modern Agricultural Industry Technology System[Hu Nong Ke Chan Zi(2024)No.9]. （2024）
