菌物学报2025，Vol.44Issue(4)：123-135,13.DOI:10.13346/j.mycosystema.240280
蛹虫草水提物及其对HaCaT细胞氧化损伤的保护作用
Cordyceps militaris aqueous extract and its protective effects against oxidative damage in HaCaT cells
孙彦庆 1张京良 2朱宗敏 1江晓路 2尚丽丽 1宗雯雯2
作者信息
- 1. 青岛黛优佳生物科技有限公司,山东青岛 266100
- 2. 青岛海洋生物医药研究院,山东青岛 266100
- 折叠
摘要
Abstract
The main active components and antioxidant activities of Cordyceps militaris aqueous extract(CME)were analyzed,and protective effects of the extract on skin cell oxidative damage in HaCaT cell model induced by hydrogen peroxide were evaluated.The results showed that CME was rich in polysaccharides,polyphenols,adenosine,cordycepin,N6-(2-hydroxyethyl)adenosine and other active substances,and could scavenge DPPH free radical,ABTS free radical and·OH free radical,with IC50 values of 0.63,2.97 and 3.24 mg/mL,respectively,indicating the CME possessed effective antioxidant activity.The CME could improve HaCaT cell survival rate through concentration-dependent manner,and the cell viability was increased from 55.39％to 83.90％at the concentration of 50 μg/mL.CME could reduce the intracellular malondialdehyde(MDA)content and significantly decrease the intracellular reactive oxygen species(ROS)level by up-regulating the activity of antioxidant enzymes such as SOD,GSH-Px and CAT(P＜0.01),thereby balancing oxidation and antioxidant systems and reducing oxidative damage.This study comes to the conclusion that CME can protect skin cell avoiding oxidative damage of hydrogen peroxide,suggesting that it has the potential to be used as a natural antioxidant in cosmetics and other fields.关键词
蛹虫草水取物/过氧化氢/氧化损伤/抗氧化Key words
Cordyceps militaris aqueous extract/hydrogen peroxide/oxidative damage/antioxidant引用本文复制引用
孙彦庆,张京良,朱宗敏,江晓路,尚丽丽,宗雯雯..蛹虫草水提物及其对HaCaT细胞氧化损伤的保护作用[J].菌物学报,2025,44(4):123-135,13.
