机械制造与自动化2025，Vol.54Issue(2)：1-13,13.DOI:10.19344/j.cnki.issn1671-5276.2025.02.001
面向细胞液-液相分离的医工检测方法研究进展
Research Progress of Liquid-liquid Phase Separation and Detection Methods in Cells
摘要
Abstract
Since liquid-liquid phase separation(LLPS)plays an important role in cell life activities,and its abnormal activities may lead to the production of diseases,it is very important to explore the principle of LLPS and detect it.This paper summerizes the recent development of LLPS,and illustrates the close relationship between LLPS phenomenon in cells and human health.The generation principle of LLPS is analyzed and summarized from two aspects of thermodynamics and internal driving force of cells,which indicates that LLPS phenomenon in cells is related to free energy,chemical potential and driving effect of various substances in the body.Several LLPS detection methods are introduced,and the application of microscope and fluorescent labeling in LLPS detection is mainly discussed.The electrical impedance tomography(EIT)technology applied to the LLPS detection is proposed to meet the requirements of real-time,fast and free marker detection,and the LLPS future development is prospected as well.关键词
液-液相分离/形成原理/检测方法/电阻抗成像技术/发展应用Key words
liquid-liquid phase separation/formation principle/detection method/electrical impendance tomography/developing application分类
信息技术与安全科学引用本文复制引用
赵尉杰,朱程君,梁晋铭,刘凯,张瑛琪,姚佳烽..面向细胞液-液相分离的医工检测方法研究进展[J].机械制造与自动化,2025,54(2):1-13,13.基金项目
国家自然科学基金面上项目(62471225) （62471225）
2023年度河北省中央引导地方科技发展资金项目(236Z7731G) （236Z7731G）
